Chromatographic Determination of Phenolics in Brassica juncea

Anubhuti Sharma; Arun Kumar; Hari Singh  Meena; Dhiraj Singh

doi:10.14233/ajchem.2017.20171

Issue

Vol. 29 No. 2 (2017): Vol 29 Issue 2

Issue Published : November 30, 2016

This work is licensed under a Creative Commons Attribution 4.0 International License.

Chromatographic Determination of Phenolics in Brassica juncea

https://doi.org/10.14233/ajchem.2017.20171

Anubhuti Sharma

ICAR-Directorate of Rapeseed Mustard Research, Sewar, Bharatpur-321 303, India

Arun Kumar

ICAR-Directorate of Rapeseed Mustard Research, Sewar, Bharatpur-321 303, India

Hari Singh Meena

ICAR-Directorate of Rapeseed Mustard Research, Sewar, Bharatpur-321 303, India

Dhiraj Singh

ICAR-Directorate of Rapeseed Mustard Research, Sewar, Bharatpur-321 303, India

Corresponding Author(s) : Anubhuti Sharma

sharmaanubhuti@yahoo.com

Asian Journal of Chemistry, Vol. 29 No. 2 (2017): Vol 29 Issue 2
Article Published : November 30, 2016

Abstract

Brassica juncea (Indian mustard) is an annual growing perennial herb. It contains various secondary metabolites which provide protective effects of the seeds. The objective of the present study was to perform the qualitative and quantitative phytochemical analysis of mustards seeds. Thin layer chromatography analysis identified six different phenolic compounds in all the genotypes. HPLC analysis also observed gallic acid, ferulic acid, sinapic acid, p-coumaric acid, p-hydroxy benzoic acid and caffeic acids as the major phenolic compounds in Brassica juncea. Sinapic acid was predominant compound among all the samples ranged from 13.42 to 15.73 mg/g DW. FA-305, FA-319, FA-337, FA-340, FA-348 showed all the six phenolic compounds with good amount of sinapic acid (14.03-15.73 mg/g DW) and gallic acid (7.26-7.76 mg/g DW). The results achieved will be used as a future source of the neutraceutical compounds and will be helpful to assess the therapeutic potential of seed extract for future pharmacological research.

Keywords

High performance liquid chromatography Brassica juncea TLC Phenolic compounds

Sharma, A., Kumar, A., Meena, H. S. ., & Singh, D. (2016). Chromatographic Determination of Phenolics in Brassica juncea. Asian Journal of Chemistry, 29(2), 296–300. https://doi.org/10.14233/ajchem.2017.20171

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References

M. Martínez-Ballesta, D.A. Moreno-Fernández, D. Castejón, C. Ochando, P.A. Morandini and M. Carvajal, Front. Plant Sci., 6, 524 (2015).

M.E. Cartea, M. Francisco, P. Soengas and P. Velasco, J. Econom. Entomol., 103, 1866 (2010).

L. Figueroa-Valverde, F. Díaz-Cedillo, E. García-Cervera, E. Pool- Gómez, M. López-Ramos, M. Rosas-Nexticapa, L. Hau-Heredia, B. Sarabia-Alcocer and G. Marquez-Estrella, Asian J. Chem., 28, 1 (2016).

D.M. Pereira, P. Valentão, J.A. Pereira and P.B. Andrade, Molecules, 14, 2202 (2009).

F. Shahidi and M. Naczk, Phenolics in Food and Nutraceuticals, CRC Press, USA, pp. 1-539 (2004).

S. Chanda and R. Nair, Indian J. Pharmacol., 38, 142 (2006).

A. Uzer, E. Ercag and R. Apak, Anal. Chim. Acta, 534, 307 (2005).

Y. Pico and C. Kozmutza, Anal. Bioanal. Chem., 389, 1805 (2007).

Zia-ur-Rehman, Food Chem., 99, 450 (2006).

S. Demiray, M.E. Pintado and L.M.P. Castro, World Acad. Sci. Eng. Technol., 54, 312 (2009).

S. Teh and J. Birch, Ultrason. Sonochem., 21, 346 (2014).

R. Sahu and J. Saxena, World J. Pharm. Res., 3, 740 (2014).

S. Nishaa, Tuberous Rhizomes.5(4) (2012) ISSN-0974-2441.

P.M. Parikh, T. Raja, L. Mula-Hussain, R. P. Baral, P. Ingle, P. Narayanan, N. Tsikai, M. O. Baki, N. Satyapal, K. O. Adusei, A. Popoola, A. Musibi, E. Nyaim, U. Tsomo, C. Opio, A. Jamshed and P. Reddy, South Asian J. Cancer, 3, 128 (2014).

K. Talreja and A. Moon, Int. J. Life Sci. Pharma Res., 4, L1 (2014).

N. Lavid, A. Schwartz, O. Yarden and E. Tel-Or, Planta, 212, 323 (2001).

M.E. Cartea, M. Francisco, P. Soengas and P. Velasco, Molecules, 16, 251 (2010).

S.K. Khetan, Endocrine Disruptors in the Environment, Wiley, pp. 109-115 (2014).

J. Gabriel, Holistic Beauty from the Inside Out: Your Complete Guide to Natural Health, Nutrition and Skincare, Seven Stories Press, pp. 31-37 (2013).

R. Bharti and T. Parvin, RSC Adv., 5, 66833 (2015).

S. Mayengbam, A. Aachary and U. Thiyam-Holländer, Antioxidants, 3, 544 (2014).

A Siger, J. Czubinski, K. Dwiecki, P. Kachlicki and M. Nogala-Kalucka, Eur. J. Lipid Sci. Technol., 115, 1130 (2013).

C. Milkowski and D. Strack, Planta, 232, 19 (2010).

S. Ou and K.C. Kwok, J. Sci. Food Agric., 84, 1261 (2004).

C.-J. Liu, Biofuels, 1, 745 (2010).

M.-R. Marton and V. Lavric, U.P.B. Sci. Bull., Series B, 75, 63 (2013).

M. Karamac, A. Kosinka, A. Rybarczyk and R.B. Pegg, Pol. J. Food Nutr. Sci., 57, 87 (2007).

S.-C. Yang, A.M. Valan, J.-H. Chun, Y.-S. Jang, Y.-H. Lee, I.H. Kim, K.-T. Lee, S.-T. Hong and S.-J. Kim, J. Agric. Chem. Environ., 4, 14 (2015).

E. Oskoueian, N. Abdullah, R. Hendra and E. Karimi, Int. J. Mol. Sci., 12, 8610 (2011).

Issue

Vol. 29 No. 2 (2017): Vol 29 Issue 2

Chromatographic Determination of Phenolics in Brassica juncea

Corresponding Author(s) : Anubhuti Sharma

Abstract

Keywords

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