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Vol. 29 No. 2 (2017): Vol 29 Issue 2

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Determination and Optimization of Vitamin B Complex (B1, B2, B3 and B6) in Cellulase Treated Polished Rice by HPLC with UV Detector

https://doi.org/10.14233/ajchem.2017.20214
Anjineyulu Kothakota
Department of Post Harvest Process and Food Engineering, College of Technology, G.B Pant University of Agriculture and Technology, Pantnager-26 3145, India
Ravi Pandiselvam
Department of Physiology, Biochemistry and Post Harvest Technology Division, ICAR-Central Plantation Crops Research Institute, Kasaragod-671 124, India
G.K. Rajesh
Department of Food and Agricultural Process Engineering, Kelappaji College of Agricultural Engineering Technology, Kerala Agricultural University, Thavanur-679 573, India
K. Gowthami
Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal-148 106, India

Corresponding Author(s) : Anjineyulu Kothakota

kothakotaanjanikumar23@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 2 (2017): Vol 29 Issue 2

Abstract

A novel processing technology is developed to polish rice in a more selective way with the help of cellulase enzyme of microbial sources. The enzyme were produced from Trichoderma reese, MTCC164. Brown rice was treated with enzyme concentration of 60-100 % (40 mL of buffer-undiluted) for 30 to 150 min (with variation of 30 min) at 30 to 50 °C (with variation of 5 °C) to attain a saturated moisture level. Selective degradation of bran layers in enzymatic polished rice (polishing time 20-100 s with variation of 20 s) has facilitated the retention of more thiamine, riboflavin, pyridoxine and niacin nutrients compared to normal milled rice. The retention of vitamin B complex nutrients detected through HPLC with UV detector and optimized by response surface methodology (RSM) with central composite design. The optimized enzyme treated bio-polished rice better sources of thiamine (51 %), riboflavin (48 %), pyridoxine (90 %) and niacin (54 %) concentration over mechanically milled rice.

Keywords

HPLC Cellulase Vitamins Brown rice Central composite design
Kothakota, A., Pandiselvam, R., Rajesh, G., & Gowthami, K. (2016). Determination and Optimization of Vitamin B Complex (B1, B2, B3 and B6) in Cellulase Treated Polished Rice by HPLC with UV Detector. Asian Journal of Chemistry, 29(2), 385–392. https://doi.org/10.14233/ajchem.2017.20214
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