Issue

Vol. 33 No. 8 (2021): Vol 33 Issue 8, 2021

Copyright (c) 2021 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Phytochemical Analysis and Comprehensive Evaluation of Pharmacological Activities, Isolation and Characterization of Bioactive Compound from the Bark of Sterculia urens Roxb.

https://doi.org/10.14233/ajchem.2021.23277
Chaitanya Darapureddy
Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram-522502, India
K.R.S. Prasad
Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram-522502, India
Phani R.S. Ch
Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram-522502, India

Corresponding Author(s) : K.R.S. Prasad

krsprasad_fed@kluniversity.in

Asian Journal of Chemistry, Vol. 33 No. 8 (2021): Vol 33 Issue 8, 2021

Abstract

The objective of the present study is to investigate the phytochemical constituents by qualitative and quantitative analysis, pharmacological activities such as antioxidant, antidiabetic, anti-inflammatory, thrombolytic and antibacterial activities of different crude extracts from bark of Sterculia urens Roxb. Further, the preparative HPLC isolation and spectroscopic characterization of the bioactive phytochemical constituents were also carried out. Different solvents such as n-hexane, ethyl acetate, methanol and water were used to prepare the crude extracts from the bark using Soxhlet extraction apparatus. DPPH free radical scavenging assay (antioxidant), α-amylase inhibition assay (antidiabetic), albumin denaturation assay (anti-inflammatory), blood clot lysis method (thrombolytic) and well-diffusion method (antibacterial) were performed for the determination of pharmacological activities of the bark extracts. The preparative HPLC analysis was carried for the separation and purification of bioactive compounds and the identification of isolated compounds was carried using 1H NMR, 13C NMR and mass spectroscopy. The quantitative estimation studies confirmed that methanolic extract contains 7.75 ± 0.141 GAE/g of phenolic compounds, 10.47 ± 0.033 mg of QE/g of flavonoids and 8.70 ± 0.047 mg/g of terpenoids. The ethyl acetate extract contains 2.16 ± 0.126 GAE/g of phenolic compounds whereas the aqueous extract contains 16.53 ± 0.055 mg/g of saponins. High DPPH radical scavenging was observed for methanolic extract with IC50 concentration of 85.38 ± 0.213 μg/mL. The α-amylase inhibition activity with IC50 concentrations of 145.67 ± 1.87, 98.36 ± 0.47 and 194.47 ± 0.55 μg/mL for ethyl acetate, methanol and aqueous extracts respectively. The albumin denaturation inhibition activity was found to be very high for methanolic extract with IC50 values of 132.08 ± 0.13 μg/mL which is near to the standard (107.13 ± 0.13 μg/mL). The % clot lysis of the methanolic extract in thrombolytic activity was found to be similar to the 100 μL of streptokinase (62.36 ± 0.140 %). Two terpenoids (One known terpenoid mansonone G and one new terpenoid) were isolated from the methanolic extract using preparative HPLC separation. Three known flavonoids (farrerol, apigenin and 6-hydroxyluteolin) and one new flavonoid were also isolated from the methanolic extract. The results suggested that bark extracts of Sterculia urens Roxb. having rich phytochemical constituents with high pharmacological activities.

Keywords

Sterculia urens Roxb. Pharmacological activities Terpenoid Flavonoids Preparative HPLC Biological activities
Darapureddy, C., Prasad, K., & R.S. Ch, P. (2021). Phytochemical Analysis and Comprehensive Evaluation of Pharmacological Activities, Isolation and Characterization of Bioactive Compound from the Bark of Sterculia urens Roxb. Asian Journal of Chemistry, 33(8), 1950–1956. https://doi.org/10.14233/ajchem.2021.23277
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. A. Bhargava, P. Shrivastava and A. Tilwari, Future J. Pharm. Sci., 7, 1 (2021); https://doi.org/10.1186/s43094-020-00150-x
  2. X.X. Huang, M. Bai, L. Zhou, L.L. Lou, Q.B. Liu, Y. Zhang, L.Z. Li and S.J. Song, J. Agric. Food Chem., 63, 7252 (2015); https://doi.org/10.1021/acs.jafc.5b02835
  3. A.G. Atanasov, B. Waltenberger, E.M. Pferschy-Wenzig, T. Linder, C. Wawrosch, P. Uhrin, V. Temml, L. Wang, S. Schwaiger, E.H. Heiss, J.M. Rollinger, D. Schuster, J.M. Breuss, M.D. Mihovilovic, B. Kopp, V. Bochkov, R. Bauer, V.M. Dirsch and H. Stuppner, Biotechnol. Adv., 33, 1582 (2015); https://doi.org/10.1016/j.biotechadv.2015.08.001
  4. N. Akhtar, Ihsan-ul-Haq and B. Mirza, Arab. J. Chem., 11, 1223 (2018); https://doi.org/10.1016/j.arabjc.2015.01.013
  5. T. Kanoje and K. Sharma, Int. J. Dev. Res., 7, 14185 (2017).
  6. M.O. Ilomuanya, R.F. Elesho, A.N. Amenaghawon, A.O. Adetuyi, V. Velusamy and A.S. Akanmu, Future J. Pharm. Sci., 6, 1 (2020); https://doi.org/10.1186/s43094-019-0015-8
  7. M.M. Gouda, R.A. Shabaraya and S.M.S. Kumar, Int. Curr. Pharm. J., 1, 376 (2012); https://doi.org/10.3329/icpj.v1i11.12064
  8. J. Redfern, M. Kinninmonth, D. Burdass and J. Verran, J. Microbiol. Biol. Educ., 15, 45 (2014); https://doi.org/10.1128/jmbe.v15i1.656
  9. A. Kathirvel and V. Sujatha, Arab. J. Chem., 9, 1435 (2016); https://doi.org/10.1016/j.arabjc.2012.03.018
  10. J.B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Chapman & Hall Ltd.: London (1973).
  11. E. Iqbal, K.A. Salim and L.B.L. Lim, J. King Saud Univ. Sci., 27, 224 (2015); https://doi.org/10.1016/j.jksus.2015.02.003
  12. R. Maria, M. Shirley, C. Xavier, S. Jaime, V. David, S. Rosa and D. Jodie, J. King Saud Univ. Sci., 30, 500 (2018); https://doi.org/10.1016/j.jksus.2017.03.009
  13. H. Noreen, N. Semmar, M. Farman and J.S.O. McCullagh, Asian Pac. J. Trop. Med., 10, 792 (2017); https://doi.org/10.1016/j.apjtm.2017.07.024
  14. S.S. Pawar and D. Dasgupta, J. King Saud Univ. Sci., 30, 293 (2018); https://doi.org/10.1016/j.jksus.2016.09.002
  15. A.M. Adeosun, S.O. Oni, O.M. Ighodaro, O.H. Durosinlorun and O.M.Oyedele, J. Taibah Univ. Med. Sci., 11, 1 (2016); https://doi.org/10.1016/j.jtumed.2015.11.006
  16. R. Batool, M.R. Khan, M. Sajid, S. Ali and Z. Zahra, BMC Chem., 32, 32 (2019); https://doi.org/10.1186/s13065-019-0549-z
  17. D.D. Sylvie, P.C. Anatole, B.P. Cabral and P.B. Veronique, Asian Pac. J. Trop. Biomed., 4, 625 (2014); https://doi.org/10.12980/APJTB.4.201414B168
  18. A.K. Shettar, M.K. Sateesh, B.B. Kaliwal and A.B. Vedamurthy, S. Afr. J. Bot., 111, 202 (2017); https://doi.org/10.1016/j.sajb.2017.03.014
  19. S. Murthuza and B.K. Manjunatha, Beni-Suef Univ. J. Basic Appl. Sci., 7, 719 (2018); https://doi.org/10.1016/j.bjbas.2018.10.001
  20. I. Hasan, S. Hussain, S. Millat, N. Sen, A. Rahman, A. Rahman, S. Islam and M.R. Moghal, J. Tradit. Complement. Med., 8, 107 (2018); https://doi.org/10.1016/j.jtcme.2017.03.005
  21. S.J. B. Dalir, H. Djahaniani, F. Nabati and M. Hekmati, Heliyon, 6, e03624 (2020); https://doi.org/10.1016/j.heliyon.2020.e03624
  22. I. Jallali, P. waffo Téguo, A. Smaoui, J.-M. Mérillon, C. Abdelly and R. Ksouri, Arab. J. Chem., 13, 2680 (2020); https://doi.org/10.1016/j.arabjc.2018.06.020
  23. P.H. Morin, M. Caude, H. Richard and R. Rosset, J. Chromatogr. A, 363, 37 (1986); https://doi.org/10.1016/S0021-9673(00)88991-8
  24. B.-Y. Hu, D.-P. Qin, S.-X. Wang, J.-J. Qi and Y.-X. Cheng, Molecules, 23, 3239 (2018); https://doi.org/10.3390/molecules23123239
  25. T.Y. Kristoferson Lulan, S. Fatmawati, M. Santoso and T. Ersam, Free Radic. Antioxid., 8, 96 (2018); https://doi.org/10.5530/fra.2018.2.15
  26. T. Murningsih, P. Praptiwi, L. Liana and A.M. Fathoni, Nusantara Bioscience, 11, 44 (2019); https://doi.org/10.13057/nusbiosci/n110108
  27. U.P.N. Kannaian, J.B. Edwin, V. Rajagopal, S.N. Shankar and B. Srinivasan, Heliyon, 6, e03411 (2020); https://doi.org/10.1016/j.heliyon.2020.e03411
  28. U. Etxeberria, A.L. de la Garza, J. Campión, J.A. Martínez and F.I. Milagro, Expert Opin. Ther. Targets, 16, 269 (2012); https://doi.org/10.1517/14728222.2012.664134
  29. M.C.N. Picot and M.F. Mahomoodally, Pharm. Biol., 55, 864 (2017); https://doi.org/10.1080/13880209.2016.1277765
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0