Issue

Vol. 33 No. 8 (2021): Vol 33 Issue 8, 2021

Copyright (c) 2021 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Antithrombotic, Antihemolytic Activities and Protein Conjugation Properties of Silver Nanoparticles Synthesized from Turbinaria ornata

https://doi.org/10.14233/ajchem.2021.23237
Amalan Venkatesan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram-608002, India
Jose Vinoth Raja Antony Samy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram-608002, India
Rajeswari Ranga Anantha Sayanam
Department of Biochemistry, Vinayaka Mission's Kirupananda Variyar Medical College and Hospitals, Vinayaka Mission′s Research Foundation (Deemed to be University), Salem-636308, India
Jayaprakash Rajendran
Department of Chemistry, School of Arts and Science, Aarupadai Veedu Campus, Vinayaka Mission’s Research Foundation-Deemed to be University, Paiyanur-603104, India
Vijayakumar Natesan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram-608002, India

Corresponding Author(s) : Vijayakumar Natesan

nvkbiochem@yahoo.co.in

Asian Journal of Chemistry, Vol. 33 No. 8 (2021): Vol 33 Issue 8, 2021

Abstract

Green synthesis of silver nanoparticles synthesized from Turbinaria ornata weed extract was carried out. Because these seaweeds are largely unexplored and contain botanical molecules that promote bimolecular reduction. In this study, silver nanoparticles (AgNPs) cause a rise in the accumulation of clotting factors, platelets and procoagulant activity, both of which lead to thrombotic and hemolytic diseases. The silver nanoparticles were initially characterized using spectroscopic analysis to validate the biomolecules of Turbinaria ornata involved in nanoparticle reduction. A detailed study on the effectiveness of silver nanoparticles on antithrombolytic activity in adult blood samples confirms the interaction of nanoparticles with platelets and blood vessels, which is significant in the production of thrombosis and cardiovascular diseases. The antihemolytic activity was tested to assess the percentage of blood clot lysis in order to confirm the ability to scavenge hydrogen peroxide in a concentration dependent manner. The protein conjugation and binding activities of major, secondary, and tertiary structures were also investigated in silico. Thus, Turbinaria ornata seaweeds with reduced silver nanoparticles demonstrated increased antithrombotic and antihemolytic activities, suggested that they could be used as novel therapeutics for cardiovascular diseases.

Keywords

Silver nanoparticles Antithrombotic Antihemolytic Turbinaria ornata Bioreduction
Venkatesan, A., Vinoth Raja Antony Samy, J., Ranga Anantha Sayanam, R., Rajendran, J., & Natesan, V. (2021). Antithrombotic, Antihemolytic Activities and Protein Conjugation Properties of Silver Nanoparticles Synthesized from Turbinaria ornata. Asian Journal of Chemistry, 33(8), 1736–1742. https://doi.org/10.14233/ajchem.2021.23237
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Miscellany, Eur. J. Phycol., 42(sup1), 23 (2007); https://doi.org/10.1080/09670260701440760
  2. J.K. Patra, G. Das, L.F. Fraceto, E. Campos, M. Rodriguez-Torres, L.S. Acosta-Torres, L.A. Diaz-Torres, R. Grillo, M.K. Swamy, S. Sharma, S. Habtemariam and H.S. Shin, J. Nanobiotechnology, 16, 71 (2018); https://doi.org/10.1186/s12951-018-0392-8
  3. K.A. Leiss, Y.H. Choi, R. Verpoorte and P.G.L. Klinkhamer, Phytochem. Rev., 10, 205 (2010); https://doi.org/10.1007/s11101-010-9175-z
  4. N. Tyagi and S.S. Kumar, Int. J. Res. Dev. Pharm. Life Sci., 6, 7 (2017).
  5. P. Deepak, R. Sowmiya, G. Balasubramani and P. Perumal, J. Taibah Univ. Med. Sci., 12, 329 (2017); https://doi.org/10.1016/j.jtumed.2017.02.002
  6. K. Chakraborty, N.K. Praveen, K.K. Vijayan and G.S. Rao, Asian Pac. J. Trop. Biomed., 3, 8 (2013); https://doi.org/10.1016/S2221-1691(13)60016-7
  7. S.S. Dakshayani, M.B. Marulasiddeshwara, M.N.S. Kumar, G. Ramesh, P.R. Kumar, S. Devaraja and R. Hosamani, Int. J. Biol. Macromol., 131, 787 (2019); https://doi.org/10.1016/j.ijbiomac.2019.01.222
  8. R.N. Krishnaraj and R.N. Berchmans, RSC Adv., 3, 8953 (2013); https://doi.org/10.1039/C3RA41246F
  9. P. Mohanpuria, N. Rana and S.K. Yadav, J. Nanopart. Res., 10, 507 (2008); https://doi.org/10.1007/s11051-007-9275-x
  10. S. Shrivastava, T. Bera, S.K. Singh, G. Singh, P. Ramachandrarao and D. Dash, ACS Nano, 3, 1357 (2009); https://doi.org/10.1021/nn900277t
  11. N.K. Hante, C. Medina and M.J. Santos-Martinez, Front. Cardiovasc. Med., 6, 139 (2019); https://doi.org/10.3389/fcvm.2019.00139
  12. B. Aslam, W. Wang, M.I. Arshad, M. Khurshid, S. Muzammil, M.H. Rasool, M.A. Nisar, R.F. Alvi, M.A. Aslam, M.U. Qamar, M.K.F. Salamat and Z. Baloch, Infect. Drug Resist., 11, 1645 (2018); https://doi.org/10.2147/IDR.S173867
  13. N. Karaki, C. Sebaaly, N. Chahine, T. Faour, A. Zinchenko, S. Rachid and H. Kanaan, J. Appl. Pharm. Sci., 3, 43 (2013); https://doi.org/10.7324/JAPS.2013.30208
  14. J. Laloy, V. Minet, L. Alpan, F. Mullier, S. Beken, O. Toussaint, S. Lucas and J.-M. Dogné, Nanomedicines, 1, 1 (2014); https://doi.org/10.5772/59346
  15. A. Lateef, M.A. Akande, S.A. Ojo, B.I. Folarin, E.B. Gueguim-Kana and L.S. Beukes, 3 Biotech, 6, 140 (2016); https://doi.org/10.1007/s13205-016-0459-x
  16. A. Gabizon, D. Goren, R. Cohen and Y. Barenholz, J. Control. Rel., 30, 53 (1998); https://doi.org/10.1016/s0168-3659(97)00261-7
  17. K.P. Kumar, W. Paul and C.P. Sharma, J. Bionanosci., 2, 144 (2012); https://doi.org/10.1007/s12668-012-0044-7
  18. N.A.N. Mohamad, N.A. Arham, J. Junaidah, A. Hadi and S.A. Idris, IOP Conf. Series: Mater. Sci. Eng. C., 358, 012063 (2017); https://doi.org/10.1088/1757-899X/358/1/012063
  19. P. Deepak, R. Sowmiya, R. Ramkumar, G. Balasubramani, D. Aiswarya and P. Perumal, Artificial Cells, Int. J. Nanomed., 45, 990 (2016); https://doi.org/10.1080/21691401.2016.1198365
  20. S. Prasad, R.S. Kashyap, J.Y. Deopujari, H.J. Purohit, G.M. Taori and H.F. Daginawala, Thromb. J., 4, 14 (2006); https://doi.org/10.1186/1477-9560-4-14
  21. E. Jun, K. Lim, K. Kim, O. Bae, J. Noh, K. Chung and J.-H. Chung, Nanotoxicology, 5, 157 (2011); https://doi.org/10.3109/17435390.2010.506250
  22. N.N. Hussein, Biochem. Cell. Arch., 18, 1721 (2019).
  23. S.R. Vijayan, P. Santhiyagu, M. Singamuthu, N.K. Ahila, R. Jayaraman and K. Ethiraj, Sci. World J., 2014, 938272 (2014); https://doi.org/10.1155/2014/938272
  24. S. Pirtarighat, M. Ghannadnia and S. Baghshahi, J. Nanostruct. Chem., 9, 1 (2019); https://doi.org/10.1007/s40097-018-0291-4
  25. R.R.R. Kannan, W.A. Stirk and J. Staden, S. Afr. J. Bot., 86, 1 (2013); https://doi.org/10.1016/j.sajb.2013.01.003
  26. A.M. Awwad, N.M. Salem and A.O. Abdeen, Int. J. Ind. Chem., 4, 29 (2013).
  27. M.A. da Silva, M.F. Rossato, G. Trevisan, C.I.B. Walker, C.A.M. Leal, D.O. Borges, M.R.C. Schetinger, R.N. Moresco, M.M.M.F. Duarte, A.R.S. dos Santos, P.R.N. Viecili and J. Ferreira, J. Evid. Based Complemen. Alternat. Med., 2012, 954748 (2011); https://doi.org/10.1155/2012/954748
  28. S. Audomkasok, W. Singpha, S. Chachiyo and V. Somsak, J. Pathogens, 2014, 203154 (2014); https://doi.org/10.1155/2014/203154
  29. M. Khalili, M.A. Ebrahimzadeh and Y. Safdari, Arh. Hig. Rada Toksikol., 65, 399 (2014); https://doi.org/10.2478/10004-1254-65-2014-2513
  30. A. Raja, S.M. Salique, P. Gajalakshmi and A. James, Int. J. Pharm. Sci. Nanotechnol., 9, 1 (2016); https://doi.org/10.37285/ijpsn.2016.9.1.6
  31. P. Appaiah and P. Vasu, J. Proteomics Bioinform., 9, 11 (2016); https://doi.org/10.4172/jpb.1000417
  32. N. Ravooru, S. Ganji, N. Sathyanarayanan and H.G. Nagendra, Front. Genet., 5, 8 (2014); https://doi.org/10.3389/fgene.2014.00291
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 2 Download : 0