A New Spectrophotometric Determination of Adrenaline with CDTA

Abstract

Adrenaline is an hormone secreted by the medulla part of the adrenal gland. Adrenaline is estimated by a new and direct spectrophotometric method after developing a colour with ammonium molybdate, sodium nitrite in presence of cyclohexylene 1,2-dinitrilotetraacetic acid (CDTA) in aqueous medium. Various experimental parameters that affect the colour saturation, like pH, time stability of the colour developed, concentration of reactants, order of addition, were studied and optimised. Interference due to many foreign ions in the estimation of adrenaline was also studied and tolerance limits were determined.