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Vol. 32 No. 5 (2020): Vol 32 Issue 5

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Cytotoxic Sterols from Philippine Mushrooms

Consolacion Y. Ragasa
Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila, Philippines
Glenn G. Oyong
Molecular Science Unit Laboratory Center for Natural Science and Environmental Research, De la Salle University, Manila, Philippines
Maria Carmen S. Tan
Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila, Philippines
Mariquit M. De Los Reyes
Department of Biology, De La Salle University, 2401 Taft Avenue, Manila, Philippines
Maria Ellenita G. De Castro
Department of Forest Biological Sciences, College of Forestry and Natural Resources (CFNR), UP Los Banos, Laguna

Asian Journal of Chemistry, Vol. 32 No. 5 (2020): Vol 32 Issue 5

Abstract

Ergosterol peroxide (1) and ergosterol (2) were commonly isolated as the major compounds of Philippine mushrooms. Sterols 1 and 2 from the dichloromethane extract of Geastrum triplex and Termitomyces clypeatus, respectively, were evaluated for their cytotoxic activities against four human cancer cell lines, viz., breast cancer (MCF-7), colon cancer (HT-29), leukemia (THP-1), and small lung cell carcinoma (H69PR), and a human normal cell line, human dermal fibroblast-neonatal (HDFn), using the PrestoBlue® cell viability assay. Compounds 1 and 2 exhibited the strongest activities against HT-29 with IC50 values of 1.79 and 2.98 μg/mL, respectively, while Zeocin gave an IC50 of 4.89 μg/mL. These compounds also exhibited strong antiproliferative effects against MCF-7 with IC50 values of 4.13 for 1 and 4.20 μg/mL for compound 2, comparable to Zeocin with IC50 = 3.68 μg/mL. Only moderate cytotoxicity resulted when compounds 1 and 2 were tested against H69PR with IC50 values of 7.78 and 6.83 μg/mL, respectively, while Zeocin exhibited an IC50of 9.81 μg/mL. Furthermore, compounds 1 and 2 showed no effects against THP-1 (IC50 > 100 μg/mL), while Zeocin showed an IC50 of 4.73 μg/mL. Although compounds 1 and 2 have been reported to exhibit different bioactivities in previous studies, the cancer cell lines tested and/or the polarities of the solvents for extraction varied. Therefore, comparisons of the cytotoxic activities of compounds 1 and 2 with earlier studies could not be made extensively.

Keywords

Cytotoxicity Ergosterol Peroxide Ergosterol Mushrooms
Ragasa, C. Y., Oyong, G. G., S. Tan, M. C., Los Reyes, M. M. D., & De Castro, M. E. G. (2020). Cytotoxic Sterols from Philippine Mushrooms. Asian Journal of Chemistry, 32(5), 1197–1202. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/2509
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References
  1. C.Y. Ragasa, M.C.S. Tan, M.E.G. De Castro and C.-C. Shen, Der Pharm. Chem., 8, 168 (2016).
  2. C.Y. Ragasa, M.C.S. Tan, M.E.G. De Castro, J. Perez and C.-C. Shen, Int. J. Toxicol. Pharmacol. Res., 8, 421 (2016).
  3. C.Y. Ragasa, M.C.S. Tan, M.E.G. De Castro and I.A. van Altena, Der Pharm. Chem., 8, 222 (2016).
  4. C.Y. Ragasa, M.C.S. Tan, R. Brkljaèa and S. Urban, Int. J. Curr. Pharm. Rev. Res., 7, 334 (2016).
  5. C.Y. Ragasa, J.M.A. Reyes, M.C.S. Tan, R. Brkljaèa and S. Urban, Int. J. Pharm. Clin. Res., 8, 1451 (2016e).
  6. N.G.U. Resurreccion, C.-C. Shen and C.Y. Ragasa, Der Pharm. Lett., 8, 117 (2016).
  7. C.Y. Ragasa, M.C.S. Tan, J. Ting, R.G. Reyes, R. Brkljaèa and S. Urban, Der Pharm. Chem., 8, 343 (2016).
  8. C.Y. Ragasa, V.D. Ebajo Jr., R.G. Reyes, R. Brkljaèa and S. Urban, Der Pharm. Chem., 7, 331 (2015).
  9. M.M. De Los Reyes, G.G. Oyong, V.D. Ebajo Jr., C.-C. Shen and C.Y. Ragasa, Asian J. Chem., 30, 613 (2018); https://doi.org/10.14233/ajchem.2018.21053
  10. R.I. Freshney, Culture of Animal Cells: A Manual of Basic Techniques. Wiley-Liss, Inc.: New York, U.S.A. (2000).
  11. M.M. De Los Reyes, G. Oyong, V. Jr, V. Ng, C.-C. Shen and C. Ragasa, J. Appl. Pharm. Sci., 5, 23 (2015); https://doi.org/10.7324/JAPS.2015.501104
  12. J. Zhang, Y. Gu and B. Chen, OncoTargets Ther., 12, 1937 (2019); https://doi.org/10.2147/OTT.S191621
  13. R.L. Geran, N.H. Greenberg, M.M. McDonald, A.M. Schumacher and B.J. Abbott, Cancer Chemother. Rep., 3, 17 (1972).
  14. S.D. Jacinto, E.A.C. Chun, A.S. Montuno, C.-C. Shen, D.L. Espineli and C.Y. Ragasa, Nat. Prod. Commun., 6, 803 (2011); https://doi.org/10.1177/1934578X1100600614
  15. C.Y. Ragasa, Manila J. Sci., 11, 42 (2018).
  16. L. He, W. Shi, X. Liu, X. Zhao and Z. Zhang, Int. J. Mol. Sci., 19, 3935 (2018); https://doi.org/10.3390/ijms19123935
  17. W. Tan, M. Pan, H. Liu, H. Tian, Q. Ye and H. Liu, OncoTargets Ther., 10, 3467 (2017); https://doi.org/10.2147/OTT.S139009
  18. J.-H. Kang, J.-E. Jang, S.K. Mishra, H.-J. Lee, C.W. Nho, D. Shin, M. Jin, M.K. Kim, C. Choi and S.H. Oh, J. Ethnopharmacol., 173, 303 (2015); https://doi.org/10.1016/j.jep.2015.07.030
  19. A. Russo, V. Cardile, M. Piovano, S. Caggia, C.L. Espinoza and J.A. Garbarino, Chem. Biol. Interact., 184, 352 (2010); https://doi.org/10.1016/j.cbi.2010.01.032
  20. M.M. Martínez-Montemayor, T. Ling, I.J. Suárez-Arroyo, G. OrtizSoto, C.L. Santiago-Negrón, M.Y. Lacourt-Ventura, A. ValentínAcevedo, W.H. Lang and F. Rivas, Front. Pharmacol., 10, 115 (2019); https://doi.org/10.3389/fphar.2019.00115
  21. Q.P. Wu, Y.Z. Xie, Z. Deng, X.M. Li, W. Yang, C.-W. Jiao, L. Fang, S.- Z. Li, H.-H. Pan, A.J. Yee, D.Y. Lee, C. Li, Z. Zhang, J. Guo and B.B. Yang, PLoS One, 7, e44579 (2012); https://doi.org/10.1371/journal.pone.0044579
  22. Y.H. Rhee, S.J. Jeong, H.-J. Lee, H.-J. Lee, W. Koh, J.H. Jung, S.-H. Kim and K. Sung-Hoon, BMC Cancer, 12, 28 (2012); https://doi.org/10.1186/1471-2407-12-28
  23. S. Shao, M. Hernandez, J.K. Kramer, D.L. Rinker and R. Tsao, J. Agric. Food Chem., 58, 11616 (2010); https://doi.org/10.1021/jf102285b
  24. Y. Yazawa, M. Yokota and K. Sugiyama, Biol. Pharm. Bull., 23, 1298 (2000); https://doi.org/10.1248/bpb.23.1298
  25. X. Li, Q. Wu, Y. Xie, Y. Ding, W.W. Du, M. Sdiri and B.B. Yang, Oncotarget, 6, 17832 (2015); https://doi.org/10.18632/oncotarget.4026
  26. R.-L. Zhao and Y.-M. He, J. Ethnopharmacol., 210, 287 (2018); https://doi.org/10.1016/j.jep.2017.08.041
  27. S. Chen, T. Yong, Y. Zhang, J. Su, C. Jiao and Y. Xie, Front Chem., 5, 1 (2017).
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