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Vol. 32 No. 5 (2020): Vol 32 Issue 5

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Synthesis of Horseradish Peroxidase-Gold Nanoparticle Conjugate through Green Route

Lovnish Siyal
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India
Benu Kumar
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India
Ranjit Kumar
Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida-201313, India
Rachana Sahney
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India

Corresponding Author(s) : Rachana Sahney

rachanasahney@gmail.com; rsahney@amity.edu

Asian Journal of Chemistry, Vol. 32 No. 5 (2020): Vol 32 Issue 5

Abstract

Synthesis of horseradish peroxidase-gold nanoparticle conjugates (HRP-AuNPs) has been studied for the development of biofunctionalized gold nanoparticles (AuNPs) through biogenic route. Herein, horseradish peroxidase enzyme has been used to synthesize gold nanoparticles at room temperature in tricine buffer. The morphology and size distribution of HRP-AuNPs conjugates were obtained by different techniques including dynamic light scattering (DLS), UV-visible (UV-vis) spectrophotometry, scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). The enzyme activity of HRP-AuNP conjugate was compared with free enzyme to determine their catalytic efficiency. The results suggests that HRP-AuNP conjugates are monodisperse particles with average hydrodynamic diameter of 83.93 ± 2.1 nm, zeta potential of about -18.4 ± 1.1 mV and higher enzyme activity towards H2O2 as compared to free horseradish peroxidase. These biofunctionalized gold nanoparticles could act as tag or labeling agent for various applications.

Keywords

Horseradish peroxidase Gold nanoparticle Zeta potential Michaelis-Menten constant
Siyal, L., Kumar, B., Kumar, R., & Sahney, R. (2020). Synthesis of Horseradish Peroxidase-Gold Nanoparticle Conjugate through Green Route. Asian Journal of Chemistry, 32(5), 1243–1247. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/2504
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