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Vol. 31 No. 5 (2019): Vol 31 Issue 5

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in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration

https://doi.org/10.14233/ajchem.2019.21627
Tien Thinh Nguyen
Department of Pharmacy and Medicine, TraVinh University, TraVinh City 940000, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam
Chan Khon Huynh
Biomedical Engineering Department, Ho Chi Minh City International University, Ho Chi Minh City 700000, Vietnam
Van Thu Le
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam
Minh Dung Truong
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam
Bach Long Giang
NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
Ngoc Quyen Tran
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam; Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam; NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
https://orcid.org/0000-0003-2899-5452
Minh Thanh Vu
Institute of Chemistry and Materials, 17 Hoang Sam, CauGiay, Hanoi 400000, Vietnam

Corresponding Author(s) : Bach Long Giang

blgiang@ntt.edu.vn

Asian Journal of Chemistry, Vol. 31 No. 5 (2019): Vol 31 Issue 5

Abstract

Adjustably biodegradable materials have gained much attention in biomedical applications. Among of them, various hydrogel-based scaffolds have applied for regenerating soft and hard tissues. In this study, according to differently biological properties of gelatin or chitosan as well as biphasic calcium phosphate nanoparticles (BCPNPs), several injectable nanocomposite hydrogels (INgel) were enzymatically fabricated from a phenolic chitosan derivative (PCD), phenolic gelatin derivative (PGD) and BCPNPs. According the change of H2O2 concentration with follow-up the time, the in situ formation of INgel was varied from 35 to 80 s. The degradation rate of the nanocomposite materials significantly related to in presence of collagenase that expended from 3 days to over one month depending on amount of the formulated PCD. The BCPNPs-encapsulated PCD-PGD INgel enhanced mineralization in the simulated biofluid. Fluorescent cytotoxicity assay indicated that the INgel was fabricated from a higher amount of the PGD resulting in a significant proliferation of bone marrow mesenchymal stem cells. These preliminary results exhibited a great potential of the INgel for bone regeneration.

Keywords

Chitosan Gelatin Nanocomposite hydrogel Collagenase Bone regeneration
Nguyen, T. T., Huynh, C. K., Le, V. T., Truong, M. D., Giang, B. L., Tran, N. Q., & Vu, M. T. (2019). in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration. Asian Journal of Chemistry, 31(5), 1062–1070. https://doi.org/10.14233/ajchem.2019.21627
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