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Analytical Methods for Ionic Profile of Dialysate Iron Therapy Drug Ferric Pyrophosphate Citrate By Ion Chromatography using Suppressed and Non-Suppressed Conductivity Detection
Corresponding Author(s) : V.R. Sankar Babu
Asian Journal of Chemistry,
Vol. 32 No. 6 (2020): Vol 32 Issue 6
Abstract
In present study, ionic profile of pharmaceutical drug molecule ferric pyrophosphate citrate by ion exchange chromatography using conductivity detection was carried out. Ferric pyrophosphate citrate is an iron organic complex used for hemodialysis for the chronic kidney disease patients. The drug is used as iron supplementation to balance the iron loss during the dialysis for the kidney affected patients. Anions, cation and metal ion were analyzed by ion chromatographic method. Relevant anions sulphate, phosphate, citrate and pyrophosphate were analyzed by suppressed conductivity detection. Both isocratic and gradient separation methods were developed for the anions analysis. Cation sodium was analyzed by non-suppressed conductivity detection. Metal iron was determined by direct conductivity detection. All the analyses were done covering selectivity, precision, linearity and accuracy aspects of analysis. Calibration outcome of RSD 0.4 to 3.0% and correlation coefficient values greater than 0.999 were observed during the studies. Spiking studies were done to check the accuracy of the analysis and the recovery values ranging from 93 to 110% were observed. Around 90% ionic content of the drug molecule can be characterized using the developed ion chromatographic methods. These methods can be directly applied for the routine analysis of drug in the pharmaceutical industries.
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- J.L. Babitt and H.Y. Lin, J. Am. Soc. Nephrol., 23, 1631 (2012); https://doi.org/10.1681/ASN.2011111078
- T.M. Ali, A.M. Genina and O.M. Abo-Salem, Beni-Suef Univ. J. Basic Appl. Sci., 3, 133 (2014); https://doi.org/10.1016/j.bjbas.2014.05.007
- J.A. Sargent and S.R. Acchiardo, Blood Purif., 22, 112 (2004); https://doi.org/10.1159/000074931
- W.H. Horl, J. Am. Soc. Nephrol., 18, 382 (2007); https://doi.org/10.1681/ASN.2006080856
- D.W. Coyne, T. Kapoian, W. Suki, A.K. Singh, J.E. Moran, N.V. Dahl and A.R. Rizkala, J. Am. Soc. Nephrol., 18, 975 (2007); https://doi.org/10.1681/ASN.2006091034
- A. Gupta, N.B. Amin, A. Besarb, S.E. Vogel, G.W. Divine, J. Yee and J.V. Anandan, Kidney Int., 55, 1891 (1999); https://doi.org/10.1046/j.1523-1755.1999.00436.x
- L.H. Fell, D. Fliser and G.H. Heine, Nephrol. Dial. Transplant., 30, 1942 (2015); https://doi.org/10.1093/ndt/gfv287
- S.N. Fishbane, A.K. Singh, S.H. Cournoyer, K.K. Jindal, Paolo Fanti, C.D. Guss, V.H. Lin, R.D. Pratt and A. Gupta, Nephrol. Dial. Transpl., 30, 2019 (2015); https://doi.org/10.1093/ndt/gfv277
- M.V. Perez-Gomez, E. Gonzalez-Parra and A. Ortiz, Clin. Kidney J., 8, 576 (2015); https://doi.org/10.1093/ckj/sfv088
- F. Locatelli, V. La Milia, L. Violo, L. Del Vecchio and S. Di Filippo, Clin. Kidney J., 8, 580 (2015); https://doi.org/10.1093/ckj/sfv057
- H. Small, T.S. Stevens and W.C. Bauman, Anal. Chem., 47, 1801 (1975); https://doi.org/10.1021/ac60361a017
- D. Jenke, J. Chromatogr. Sci., 524, 49 (2011); https://doi.org/10.1093/chrsci/49.7.524
- N.H. Subramanian, S. Thyagarajan and P. Manigandan, J. Chromatogr. Sci., 47, 549 (2009); https://doi.org/10.1093/chromsci/47.7.549
- N.H. Subramanian, V.R. Sankar Babu, R.G. Jeevan and G. Radhakrishnan, J. Chromatogr. Sci., 47, 529 (2009); https://doi.org/10.1093/chromsci/47.7.529
- N.H. Subramanian, P. Manigandan, R. Ganeshjeevan, G. Radhakrishnan, A. Wille and A. Steinbach, Pharmaceutical/Drug Discovery, The Application Book, pp. 27-29 (2009)
References
J.L. Babitt and H.Y. Lin, J. Am. Soc. Nephrol., 23, 1631 (2012); https://doi.org/10.1681/ASN.2011111078
T.M. Ali, A.M. Genina and O.M. Abo-Salem, Beni-Suef Univ. J. Basic Appl. Sci., 3, 133 (2014); https://doi.org/10.1016/j.bjbas.2014.05.007
J.A. Sargent and S.R. Acchiardo, Blood Purif., 22, 112 (2004); https://doi.org/10.1159/000074931
W.H. Horl, J. Am. Soc. Nephrol., 18, 382 (2007); https://doi.org/10.1681/ASN.2006080856
D.W. Coyne, T. Kapoian, W. Suki, A.K. Singh, J.E. Moran, N.V. Dahl and A.R. Rizkala, J. Am. Soc. Nephrol., 18, 975 (2007); https://doi.org/10.1681/ASN.2006091034
A. Gupta, N.B. Amin, A. Besarb, S.E. Vogel, G.W. Divine, J. Yee and J.V. Anandan, Kidney Int., 55, 1891 (1999); https://doi.org/10.1046/j.1523-1755.1999.00436.x
L.H. Fell, D. Fliser and G.H. Heine, Nephrol. Dial. Transplant., 30, 1942 (2015); https://doi.org/10.1093/ndt/gfv287
S.N. Fishbane, A.K. Singh, S.H. Cournoyer, K.K. Jindal, Paolo Fanti, C.D. Guss, V.H. Lin, R.D. Pratt and A. Gupta, Nephrol. Dial. Transpl., 30, 2019 (2015); https://doi.org/10.1093/ndt/gfv277
M.V. Perez-Gomez, E. Gonzalez-Parra and A. Ortiz, Clin. Kidney J., 8, 576 (2015); https://doi.org/10.1093/ckj/sfv088
F. Locatelli, V. La Milia, L. Violo, L. Del Vecchio and S. Di Filippo, Clin. Kidney J., 8, 580 (2015); https://doi.org/10.1093/ckj/sfv057
H. Small, T.S. Stevens and W.C. Bauman, Anal. Chem., 47, 1801 (1975); https://doi.org/10.1021/ac60361a017
D. Jenke, J. Chromatogr. Sci., 524, 49 (2011); https://doi.org/10.1093/chrsci/49.7.524
N.H. Subramanian, S. Thyagarajan and P. Manigandan, J. Chromatogr. Sci., 47, 549 (2009); https://doi.org/10.1093/chromsci/47.7.549
N.H. Subramanian, V.R. Sankar Babu, R.G. Jeevan and G. Radhakrishnan, J. Chromatogr. Sci., 47, 529 (2009); https://doi.org/10.1093/chromsci/47.7.529
N.H. Subramanian, P. Manigandan, R. Ganeshjeevan, G. Radhakrishnan, A. Wille and A. Steinbach, Pharmaceutical/Drug Discovery, The Application Book, pp. 27-29 (2009)