Spectrophotometric Methods For Simultaneous Determination of Sulphadoxine and Pyrimethamine in Two Component Tablet Formulations

Abstract

Simultaneous determination of active ingredients in multicomponent pharmaceutical products normally requires the use of separation techniques, such as HPLC or GC, followed by their quantitation. Presented here are two analytical methods, i.e., derivative spectroscopy and multiwavelength spectroscopy, that does not require prior separation for simultaneous determination of sulphadoxine and pyrimethamine in two components table formulation. Shimadzu UV 160A spectrophotometer, capable of multicomponent analysis, was used for quantitation. Determination were made in 0.1 N methanolic hydrochloric acid. Zero crossing point technique of measurementsin used in derivative spectroscopy, whereas multiwavwlength spectroscopic method is based on principal of overdetermination. Recordings of absorbances of standard solutions at 240 nm, 245 nm and 250 nm were processed by means of matrix equations and results for sample solutions were obtained. Beer’s law is obeyed in the concentration range of 0-30 mcg/mL of sulphadoxine and 0-10 mcg/mL of pyrimethamine. The utility of developed methods has been demonstrated by analysis of commercial formulation containing both the drugs. The methods have been validated stastically and were found to be satisfactory.