Dual- Wavelength Spectrophotometric Determination of Trace Amounts of Iron in Water and Food Using Copper-o-Chlorophenylfluorone Complex as Chromogenic Reagent

Abstract

The reaction between iron (Fe³⁺) and the chromogenic reagent,
o-chlorophenylfluorone (o-CPF) was sensitive and selective at pH
6 and in the presence of excess of copper(II). It was used for the
determination of trace amounts of iron by primary-secondary
wavelength spectrophotometery (PSWS). This method gave higher
sensitivity than ordinary spectrohotometery because it can utilize
simultaneously the peak absorption and the valley absorption of tire
complex solution. By analyzing several samples, the relative standard
deviations were less than 3.8% and the recovery rate of Fe
between 92.5 and 108%.