Assay of Tylosin in Pharmaceutical Formulations by Visible Spectrophotometry

Abstract

Simple spectrophotometric methods (M₁ and M₂) for the assay
of tylosin (TS) in pharmaceuticals are described. Method M₁ is
based on the formation of oxidative coupling product of tylosin
with 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the
presence of ferric chloride (Fe(III)   λ_max 640 nm). Method M₂ is
based on the formation of a coloured charge transfer complex between
TS and chloranilic acid (CA) (λ_max 520 nm). Beer's law
limits, precision and accuracy of the methods are checked by the
UV reference method. The methods are found to be suitable for the
assay of tylosin in pharmaceutical formulations. The per cent
recoveries range from 99.02 to 100.96.