Development and Validation of Spectrophotometric Methods for the Estimation of Itraconazole

Abstract

Three simple spectrophotometric methods (A, B and C) have been
developed for the determination of itraconazole in pure and in its pharmaceutical
formulations. Method A is based on the formation of blood red
colored complex with ferric chloride and 2,2' -bipyridine having absorption
maximum at 525 nm. In method B, itraconazole forms green coloured
complex with ferric chloride and potassium ferricyanide exhibiting maximum
absorption at 725 nm, whereas method C is based on the oxidation
of the drug with a known excess of oxidant. potassium permanganate
(KMnO₄). The excess permanganate is determined using the dye, Fast
Green FCF (FGFCF) at 625 nm. The results obtained are reproducible and
are statistically validated.