UV and Visible Spectrophotometric Methods for the Determination of Roxatidine Acetate Hydrochloride in Pharmaceutical Formulations

Abstract

Two simple and reproducible spectrophotometric methods have

been developed for the estimation of roxatidine acetate hydrochloride.

In the UV region, the drug showed maximum absorption at

208 nm in distilled water. In the proposed visible spectrophotometric

method, the drug is made to react with Folin-Ciocalteu (FC) reagent

under alkaline conditions, when it forms a blue coloured chromogen

having absorption maximum at 750 nm. These methods obey Beer's

law in the concentration range 5-40 μg/mL and 25-100 μg/mL

respectively. The methods are statistically evaluated for accuracy

and precision.