Spectrophotometric Estimation of Gliclazide in Pharmaceutical Dosage Forms

Abstract

Three simple and reproducible spectrophotometric methods have been developed for the estimation of gliclazide in pure and its dosage forms by using 0.1 N NaOH (method A), 0.1 N HCl (method B) and pH 7.4 phosphate buffer (method C). The maximum absorbance was observed at 226 nm, 230 nm and 226 nm in methods A, B and C respectively. Beers's law was obeyed in the concentration of 2.5–50 μg/mL, 5–60 μg/mL and 2.5–60 μg/mL in methods A, B and C respectively.