Estimation of Ondansetron Hydrochloride in Pharmaceutical Dosage Forms by HPLC

Abstract

High pressure liquid chromatographic method (HPLC) has been developed for the estimation of ondansetron hydrochloride in its pharmaceutical tablet dosage forms using RP C-18 column. The mobile phase consisted of methanol and 0.02 M phosphate buffer in the ratio of 60 : 40 and doxazocin was used as internal standard.
The detection was carried out at 213 nm and the linearity was found
to be in the range of 0.01 to 59 μg/mL. The method is simple,
precise, specific, less time consuming and accurate for the estimation
of ondansetron hydrochloride in tablet dosage forms.