Spectrophotometric Estimation of Clarithromycin in Pharmaceutical Formulations

Abstract

Two simple and sensitive spectrophotometric methods A and B have been developed for the estimation of clarithromycin in pure and pharmaceutical dosage forms. Method A is based on the reaction of the drug with iron(III) and a subsequent reaction with potassium ferricyanide to yield a prussian blue coloured product with a maximum
absorption at 740 nm. In method B, the drug forms a blue
coloured complex with Folin-Ciocalteu reagent, showing maximum
absorption at 775 nm. The methods obey Beer's law in the concentration
range of 12.5–75.0 μg/mL (method A) and 250–125.0
μg/mL (method B). The common excipients used as additives in
tablets do not interfere with the proposed methods. Analytical data
for determination of the pure compound are presented together with
the applications of the proposed methods to the analysis of some
tablets. The methods have been statistically evaluated and are found
to be precise and accurate.