Thermal and Optical Radiation Effects on Liposomes Encapsulated Haemoglobin

Abstract

Synthetic red blood cells known as liposomes encapsulated haemoglobin (LEH) is a material that offers an excellent alternative to substitution blood since the chemical composition of the liposomes encapsulated oxyhaemoglobin (Oxy LEH), is similar to that of the natural blood cells. Unfortunately, this alternative, like the natural blood, has a limited shelf life at room temperature due to thermal, and/or solar (optical) radiations exposure. In this study
experiments were run on LEH assays, with and without protection
additives. to weigh up the effect of exposing LEH to thermal effect.
using a digital controlled oven, and to optical radiations. using an
appropriate apparatus to stimulate the solar optical radiations. Constant
evaluation of the acceptability of this artificial blood alternative
was done with the help of an advanced computerized technique.
The results of the experiment have shown that exposing LEH to
thermal and optical radiations leads to a reduction of the concentration
of the oxyhaemoglobin and a' heightened of the concentration
of the methaemoglobin. The rate of LEH degradation in the two
treatments was linked to the time in which they were exposed both
to the thermal and optical radiations effects. Antioxidants. ascorbic
acid and α-tocopherol were used in order to conserve and guard
the prepared LEH from the degradation that happens to it due to
the effects of both thermal and optical radiations.