Development and Validation of a Reversed-Phase HPLC Method for the Analysis of Nimodipine in Pharmaceutical Dosage Forms
Corresponding Author(s) : Y.S.R. Krishnaiah
Asian Journal of Chemistry,
Vol. 15 No. 3 (2003): Vol 15 Issue 3
Abstract
A rapid and sensitive high-performance liquid chromatographic
method was developed for the estimation of nimodipine in pharmaceutical
dosage forms. Nimodipine was chromatographed on a reverse
phase C-18 column in a mobile phase consisting of acetonitrile
and water in the ratio of 58 : 42 (v/v). The mobile phase was pumped
at a flow rate of 1 mL/min and the eluents were monitored at 241
nm. The calibration curve was linear in the range of 0.1-40 μg/mL.
The intra- and inter-day variation was found to be less than 1 %
showing high precision of the assay method. The mean recovery of
the drug from the solution containing 10 μg/mL was 99.95 ± 0.86%
indicating high accuracy of the proposed HPLC method. Due to its
simplicity, rapidness, high precision and accuracy, the proposed
HPLC method may be used for determining nimodipine in bulk
drug samples and pharmaceutical dosage forms.
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