Reverse-Phase HPLC Method for the Estimation of Selegiline Hydrochloride in Pharmaceutical Dosage Forms

Abstract

A rapid and sensitive high-performance liquid chromatographic
method was developed for the estimation of selegiline hydrochloride
in pharmaceutical dosage forms. Selegiline hydrochloride was chromatographed
on a reverse phase C-18 column using ondansetron
hydrochloride as internal standard in a mobile phase consisting of
methanol and water in the ratio of 70:30 v/v. The mobile phase was
pumped at a flow rate of 1 mL/min, and the eluents were monitored
at 206 nm. The calibration curve was linear in the range of 0.2 to
20  μg/mL. The intra- and inter-day variation was found to be less
than 1 % showing high precision of the assay method. The mean
recovery of the drug from the solution containing 5 μg/mL was
98.47 ± 0.37% indicating high accuracy of the proposed HPLC
method. Due to its simplicity, rapidness, high precision and accuracy,
the proposed HPLC method may be used for determining
selegiline hydrochloride in bulk  drug samples or in tablets.