Development of a HPLC Method for the Estimation of Mebendazole in Human Plasma

Abstract

A HPLC method for the estimation of mebendazole in human
plasma was developed. Varying amounts of mebendazole (5 to 400
ng), fixed quantity (0.5  μg) of tinidazole (internal standard) and
0.2 mL of 20% trichloroacetic acid solution were added to drug-free
human plasma (0.5 mL). The mixture was vortexed for 5 min and
centrifuged at 3,000 rpm for 10 min and the supernatant liquid was
filtered. Twenty microlitres of the resultant filtrate was injected into
a reverse phase C-18 column using a mobile phase consisting of
acetonitrile and water (containing 0.4% triethylamine and pH
adjusted to 4.0 with 5% orthophosphoric acid) in the ratio of
38 : 62%v/v at a flow rate of 0.8 mL/min. The eluents were monitored
at 254 nm. The method was validated for its linearity, precision
and accuracy. The results of the study show that the present HPLC
method is simple, precise, specific, less time consuming and accurate
for the estimation of mebendazole in human plasma samples.