Estimation of Nimodipine in Human Plasma by HPLC Method

Abstract

A high performance liquid chromatographic (HPLC) assay was developed
for the determination of nimodipine in human plasma. Precipitation
technique was employed with a RP-HPLC separation for the analysis.
Varying amounts of nimodipine (10 to 200 ng), fixed quantity (0.5  μg) of
nimesulide (internal standard) and 0.3 mL of acetonitrile were added to
blank human plasma (0.5 mL). The mixture was vortexed for 5 min and
centrifuged at 3,000 rpm for 10 min and the supernatant liquid was filtered.
Twenty microlitres of the resultant filtrate was injected into a reverse phase
C-18 column using a mobile phase consisting of methanol and water in
the ratio of 68 : 32% v/v at a flow rate of 0.9 mL/min. The eluents were
monitored at 237 nm. The method was validated for its linearity, precision
and accuracy. The method was found to be simple, precise, sensitive, less
time consuming and accurate for the estimation of nimodipine in human
plasma.