Development of a HPLC Method for the Estimation of Celecoxib in Human Plasma

Abstract

Varying quantity of celecoxib (25 to 1500 ng) was added to
drug-free human plasma (0.5 mL) and mixed with 0.5 mL of acetonitrile.
The mixture was vortexed for 5 min and centrifuged at 3,000
rpm for 10 min and the supernatant liquid was filtered. Fifty microlitres
of the resultant filtrate was injected into a reverse phase C-18
column using a mobile phase consisting of methanol and acetic acid
(pH adjusted to 3.0 with dilute ammonia solution) in the ratio of
68 : 32% v/v at a flow rate of 1.5 mL/min. The eluents were monitored
at 254 nm. The method was validated for its linearity, precision
and accuracy. The present HPLC method was found to be
simple, precise, specific, less time consuming and accurate for the
estimation of celecoxib in human plasma.