Reverse-Phase HPLC Method for the Estimation of Ornidazole in Human Plasma

Abstract

A high-performance liquid chromatographic method was developed for
the estimation of omidazole in human plasma. Varying amounts of ornidazole
drug solution (10 to 800 ng) and 5 mL of ethyl acetate were added
to blank human plasma (0.5 mL). The mixture was vortexed for five
minutes and centrifuged at 3,000 rpm for 10 min and organic layer was
separated and dried under vacuum evaporation .The residue is reconstituted
with 0.3 mL of methanol. Twenty microliters of this solution was injected
into a reverse phase C-18 column using a mobile phase consisting of
methanol and triply distilled water (consisting of 0.4% triethylamine and
pH adjusted to 3.6 with 5% orthophosphoric acid) in the ratio of 50 : 50
v/v and the eluents were monitored at 254 nm. The method was statistically
validated for its linearity, precision and accuracy. The intra- and inter-day
variation was found to be less than 2% indicating that the method is highly
precise. Due to its simplicity, sensitivity, high precision and accuracy, the
proposed HPLC method may be used for estimation of ornidazole in
plasma.