Estimation of Tinidazole in Human Plasma by Reverse Phase HPLC Method

Abstract

A rapid and sensitive high-performance liquid chromatographic
method was developed for the estimation of tinidazole in human
plasma. Varying amounts of tinidazole (10 to 800 ng), fixed quantity
(2  μg) of mebendazole (internal standard) and 0.2 mL of 20%
perchloric acid were added to blank human plasma (0.5 mL). The
mixture was vortexed for 5 min and centrifuged at 3,000 rpm for
10 min and the supernatant liquid was separated and filtered. Twenty
microliters of the resultant filtrate was injected into a reverse phase
C-18 column using a mobile phase consisting of acetonitrile and
water (consisting of 0.4% triethylamine and pH 3.6 adjusted with
5%orthophosphoric acid) in the ratio of 44:56 v/v at a flow rate 0.8
mL/min. The eluents were monitored at 254 nm. The calibration
curve was linear in the range of 10-800 ng/0.5 mL of plasma. Due
to its simplicity, sensitivity, high precision and accuracy, the proposed
HPLC method may be used for biopharmaceutical and pharmacokinetic
evaluation of tinidazole formulations.