A High Performance Liquid Chromatographic Assay for Mosapride Citrate

Abstract

A rapid and sensitive reverse phase high performance liquid chromatographic
method has been described for the estimation of mosapride citrate
in bulk drug samples and pharmaceutical dosage forms using RP C-18
column. Nifedipine was used as internal standard. The mobile phase consisted
of methanol and water in the ratio of 73 : 27 respectively, and was
pumped at 1.3 mL/min at 40^oC. The drug and internal standard were eluted
at 4.11 min and 2.54 min respectively. The detection was carried out at
274nm and the calibration curve was linear in the range of 0.01 to 20
μg/mL. The method was statistically validated for its linearity, precision
and accuracy. The intra- and inter-day variation was found to be less than
1 % showing high precision of the assay method. The detection limit was
found to be 5  μg/mL. Due to its simplicity, rapidness, high precision and
accuracy, the proposed HPLC method may be used for determining
mosapride citrate in bulk drug samples or in pharmaceutical formulations.