Spectrophotometric Estimation of Celecoxib from Capsule Formulation

Abstract

Two simple and sensitive spectrophotometric methods have been developed for the quantitative estimation of celecoxib from its capsule formulation. The first developed method is a UV spectrophotometric method using methanol as solvent; the drug showed absorption maxima at 253.2 nm in methanol and linearity was observed in the concentration range of 5–15 μg/mL. The second developed method is a visible spectrophotometric method, based on formation of red coloured complex of drug with 1,10-phenanthroline and ferric chloride, the complex showed absorbance maxima at 509.2 nm and linearity was observed in the concentration range of 50–400 μg/mL. Results of analysis for both the developed methods were validated statistically and by recovery studies.