Identification of Ethylchloroformate Derivatives of Amino Acids and Hydrolysis of Myoglobin and Small PeptidesUsing Gas Chromatography-Mass Spectrometry

Abstract

Derivatization of twenty amino acids was investigated, using ethanol, methanol, propanol, ethyl chloroformate and pyridine, to provide volatile products which are easily amenable to GC-MS. This method presents the rapidity of sample preparation and speed of GC-MS analysis. The results of the analysis of the hydrolysis products of protein and peptides indicated that this technique could be used to identify most of the amino acid contents of peptides and proteins.