Electrochemical and Spectroscopic Studies on the Interaction between (1,10-Phenanthroline- N,N')-nickel(II) phthalate and DNA

Abstract

The crystal structure of ([Ni(phen)](phth)(H₂O).H₂O (1) [Ni(phen)]²⁺) (phen = 1,10-phenanthroline, phth = phthalate) was determined by X-ray crystallography. The interaction between [Ni(phen)]²⁺ and salmon sperm DNA in 0.2 mol L^-1 Britton Robinson buffer solution (pH 6.50) was studied by cyclic voltammetry, ultraviolet spectroscopy and fluorescence spectroscopy. All the experimental results indicate that [Ni(phen)]²⁺ can bind to DNA mainly by intercalative binding mode. The binding ratio of the DNA-[Ni(phen)]²⁺ association complex is calculated to be 1 : 1 and the binding constant is 8.28 x 10² L mol^-1.