Simultaneous Estimation of Glimepiride and Pioglitazone in Bulk and in Pharmaceutical Formulation by HPTLC Method

Abstract

A validated HPTLC method for simultaneous estimation of glimepiride and Pioglitazone in bulk and in tablet formulations is described. Separation was achieved on aluminum sheet of silica gel 60F₂₅₄ using toluene : ethyl acetate : methanol (50 : 45 : 5 v/v/v) as mobile phase. Quantification was achieved with UV detection at 230 nm over the concentration range of 200-700 ng/spot and 1500-5250 ng/spot with mean recovery of 98.40 ± 0.675 and 98.75 ± 1.140 for glimepiride and pioglitazone, respectively. R_f values for glimepiride and pioglitazone were found to be 0.49 and 0.61, respectively. The method was validated in terms of accuracy and precision. The proposed method is simple, precise, sensitive and applicable for the simultaneous determination of glimepiride and pioglitazone in bulk powder and in tablets.