Synthesis of 2,3,7-trihydroxy-9-(3,4,5-trimethoxy)- phenylflurone-3 and Its Application to Spectrofluorescence Quenching Determination of Microamounts of Molybdenum in Tobacco

Abstract

A new fluorescence reagent, namely 2,3,7-trihydroxy-9-(3,4,5-trimethoxy)phenylflurone-3 was synthesized and sensitive method for the spectrofluorescence quenching determination of molybdenum with the fluorescence reagent, in presence of cetyltrimethyl ammonium bromide, is described. In H₃PO₄ medium, the fluorescence intensity of trimethoxyphenylfluorone is quenched due to formation of complex of molybdenum and trimethoxyphenylfluorone. The detection limit is 1.5 ng mL^-1 and linear range is between 2-80 ng mL^-1. The proposed method has been applied to the determination of Mo in tobacco.