HPTLC Method for Determination of Ezetimibe in Tablets

Abstract

A simple, sensitive HPTLC method has been developed for the analysis of ezetimibe in its commercial single component tablet formulations (10 mg/tablet). The study employs a silica gel 60GF₂₅₄ on aluminium foil and a mobile phase used was a mixture of toluene: acetone (6:4 % v/v). Detection was carried out at 233 nm. The R_f value was 0.52 for ezetimibe. The linearity was observed in the range of 300-2100 ng/spot. The recovery study was carried out by standard addition method and was found to be 99-101 %.The method was validated as per ICH guidelines.