Validated Simultaneous Estimation of Ornidazole and Cefixime by RP-HPLC in Pure and Pharmaceutical Dosage Form

Abstract

A simple, precise RP-HPLC method was developed for the estimation of ornidazole and cefixime in pure and pharmaceutical dosage forms. The quantification was carried out using a C-18 column 250 × 4.6 mm i.d., 5 μm particle size in isocratic mode, with mobile phase comprising of tetrabutyl ammonium hydroxide and acetonitrile in the ratio of 3:1 (v/v) pH 6.5. The flow rate was 1.5 mL/min and the detection was carried out UV detector at 254 nm. The retention times were 4.75 and 8.98 min for cefixime and ornidazole, respectively. The method produced linear response in the concentration range of 150-350 μg/mL for ornidazole 60-140 μg/mL for cefixime, respectively. The percentage recovery was found to be 99.6 and 99.8 % for ornidazole and cefixime, respectively. Metronidazole used as an internal standard in the present study. The method validated by evaluation of required parameters.