Chromatographic Separation of Enantiomers Acids Using Quinine as Chiral Counter-Ion in Mobile Phase

Abstract

In present studies, the chromatographic behaviour of quinine as chiral counter ion and the separation of 10-camphor sulfonic acid and mandelic acid, using porous graphitic carbon Hypercarb, as stationary phase is described. The 10-camphorsulfonic acid and mandelic acid enantiomers were separated in mobile phase system consisting of dichloromethane-cyclohexane (50:50 % v/v) + 0.10 mM quinine + 0.008 % CH₃COOH, λ = 257 nm for mandelic acid, λ = 337 nm for 10-camphorsulfonic acid and 5 μL was injected into the column.