Separation of Camelliaside C from Tea Seed by RP-HPLC

Abstract

The plant Camellia sinensis has been cultivated widely in oriental countries as a source of various types of tea. The main flavonoids in these teas are glycosides of kaempferol including camelliasides. Using the pure water, the Camelliaside C contained in tea seed was extracted. The pretreatment steps were composed of solvent extraction, filtration, concentration and membrane filtration. The procedures of extraction separation were proposed and the extraction effects of various extractive solvents were compared. As a potential skin diseases agent, the Camelliaside C contained in tea seed (Korea) was considered for isolation by reversed-phase high-performance liquid chromatography. The mobile phase was linearly varied from an A/B ratio of 85/15 to 65/35 vol. % over a period of 50 min (A: water/acetic acid, 99.9/0.1 vol. % and B: acetonitrile/acetic acid, 99.9/0.1 vol. %). It was shown that Camelliaside C is contained in water and n-butanol extracts, but ethyl acetate layer did not contain the target material.