Spectrophotometric Methods for the Determination of Indinavir Sulphate

Abstract

Three simple and sensitive visible spectrophotometric   methods (A, B and C) have been developed for the estimation   of indinavir sulphate in the bulk and dosage forms. Method   A, involves the addition of excess chloramine-T of known   concentration in acidic medium to indinavir sulphate and   determination of un-reacted chloramine-T by measure of   decrease in the absorbance of dye gallocyanine (GC, λ_max 540   nm). Method B is based on the reduction of heteropolyacid,   phosphomolybdotungstic acid, (FC) reagent by indinavir   sulphate producing a characteristic intense blue colour (λ_max   760 nm). Method C is based on the charge-transfer complex   formation between indinavir sulphate and chloranil (λ_max 540   nm). Regression analysis, Beer's law plots showed good   concentration ranges of 2.5-20, 2-25 and 4.5-40 μg/mL for   methods A, B and C, respectively.