Tobacco leaf samples from different leaf positions, different aging time were extracted for the determination of organic acids. Organic acids in tobacco have been determined by RP-HPLC method combined with UV detection. The gradient mobile phase employed was 20 mM phosphate buffer (pH 2.6) and acetonitrile at a flow-rate of 1.0 mL/min. Recoveries for organic acids were in the range of 84.2 to 106.3 %. The relative standard deviation of the repeating of the migration time and peak area of five replicate analyses of 11 organic acids ranged from 0.42 to 1.78 % and from 0.47 to 2.5 %, respectively. The results show that organic acids concentrations varied with aging time and different leaf position.
Guo, J.-C., Guo, Z., Cheng, X.-L., Wang, N.-R., & Ming, N.-N. (2010). Reversed Phase HPLC Studies on the Change of the Content of Organic Acids in Tobacco During Aging. Asian Journal of Chemistry, 21(1), 605–611. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/18963
