in vitro Evaluation of Leaves and Fruits of Elaeagnus latifolia L. for Antioxidant and Antimicrobial Activities

Abstract

In this work, we have studied the antioxidant and antimicrobial potential of leaves and fruits of Elaeagnus latifolia L. Antioxidant activities were conducted by using hydrogen peroxide radical scavenging and DPPH method. Total phenolic and flavonoid content was measured by using gallic acid and quercetin equivalent. in vitro Antibacterial activity of these plants was examined against five pathogenic bacteria. Highest DPPH scavenging activity was shown by methanolic extract of the flower of Elaeagnus latifolia with an IC₅₀ value of 144.64 ± 0.25 μg mL^-1 in comparision to standard ascorbic acid with an IC₅₀ value of 29.39 ± 7.11 μg mL^-1. Highest hydrogen peroxide radical scavenging activity was shown by the leaves of Elaeagnus latifolia with an IC₅₀ value of 444.59 ± 3.77 μg mL^-1, whereas IC₅₀ value of standard ascorbic acid is 279.89 ± 1.81 μg mL^-1. The leaves of Elaeagnus latifolia showed highest total phenolic and flavonoid content with 61.15 ± 1.23 μg mL^-1 and 15.12 ± 0.125 μg mL^-1, respectively. The result of antimicrobial study showed that the plant is potent against the tested organism which is comparable to standard drug ofloxacin. From the findings, it can be interpreted that the plant could be utilized as natural source of antioxidant in food processing or in medicine industry.