Reversed-Phase HPLC Method for Determination of Quercetin in Human Plasma
Corresponding Author(s) : J.Vijaya Ratna
vijaya_ratna@yahoo.com
Asian Journal of Chemistry,
Vol. 21 No. 1 (2009): Vol 21 Issue 1
Abstract
A simple reversed-phase liquid chromatography method was developed for the quantitative determination of quercetin. The method is simple, sensitive and highly selective and involves single extraction of drug from plasma in (4:1) ratio of methanol: DMSO. The mobile phase was pumped at a flow rate of 1.0 mL/min and the effluent was monitored at 370 nm. The retention time of quercetin was 2.72 min. The limit of detection of drug in plasma was found to be 0.2 μg/mL. The method was validated for its linearity, precision and accuracy.
Keywords
Quercetin
RP-HPLC
UV Detection
Human plasma
Vijaya Sri, K., Ratna, J., Annapurna, A., & Ravi Kumar, B. (2010). Reversed-Phase HPLC Method for Determination of Quercetin in Human Plasma. Asian Journal of Chemistry, 21(1), 101–104. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/18901
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