Spectrophotometric Estimation of Metadoxine in Oral Solid Dosage Forms

Abstract

Three new, simple sensitive and reproducible spectrophotometric methods have been developed for the estimation of metadoxine in oral solid dosage form, (method A, B and C). Method A obeys Beer's law in concentration ranging from 8-64 μg/mL and exhibiting maximum absorption at 291 nm. Method B obeys Beer's law in the same concentration from 8-64 μg/mL. By derivatising the primary spectra the second derivative spectrum is obtained. Method C is difference spectroscopy method which is based on shifting the λ_max by changing the pH of the solution, by adding 0.1 M HCl and 0.1 M NaOH. This produces maximum absorption at 309, 245 and 290 nm. At 245 nm, it obeys Beer's linearity in concentration ranging from 8-64 μg/mL. The methods were extended upto pharmaceutical formulations and there was no interference from any common excipients and diluents. The results of analysis have been validated statistically by recovery studies.