Comparison of Aspirin s Antioxidant Effect with Selenium and Vitamin E by Measuring Mitochondrial Selenium Dependent Glutathione Peroxidase and Cytosolic Total Glutathione Peroxidase Activity in Mice Liver

Abstract

In this study, the antioxidant effect of aspirin was studied and compared
with selenium and vitamin E on the activities of mitochondrial selenium
dependent glutathione peroxidase (Se-GPX) and cytosolic total glutathione
peroxidase (t-GPX). After the injection of selenium, vitamin E
and aspirin intraperitonally to the mice, they were killed by servical
dislocation at the 2, 4, 8, 12 and 24 h and their livers were removed.
Enzyme fractions were obtained from livers. Mitochondrial fractions
were used to find out the changes in the Se-GPX activities and cytosolic
fractions were used to find out the changes in the total GPX activity.
Aspirin increased cytosolic t-GPX activity in 12 and 24 h and mitochondrial
Se-GPX activity in 8 h when compared with controls (p < 0.05).
The t-GPX activity was less than vitamin E in 2 h and selenium in 12 h
(p < 0.05). The study shows that aspirin has an antioxidant effect by
increasing the cytosolic t-GPX and mitochondrial SeGPX activity. The
antioxidant effect of aspirin is less than selenium and vitamin E.