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Vol. 26 No. 18 (2014): Vol 26 Issue 18

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A New Gradient Liquid Chromatographic Method for Simultaneous Estimation of Tenofovir Disoproxil Fumarate, Cobicistat, Emtricitabine and Elvitegravir in Bulk Drug and Tablet Dosage Form

https://doi.org/10.14233/ajchem.2014.17196
V.V. Raveendra Babu
Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur-313 024, India
Pankaj K. Sharma
School of Pharmaceutical Sciences, Jaipur National University, Jaipur-302 025, India
I. Singhvi
Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur-313 024, India

Corresponding Author(s) : V.V. Raveendra Babu

raveendra.vemula@gmail.com

Asian Journal of Chemistry, Vol. 26 No. 18 (2014): Vol 26 Issue 18

Abstract

A new gradient reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of tenofovir disoproxil fumarate, cobicistat, emtricitabine and elvitegravir. The chromatography was achieved on Hypersil BDS, C18 column, (100 × 4.6 mm, 5 m) with a mobile phase composed of phosphate buffer (pH 3) and acetonitrile taken in gradient mode at a flow rate of 0.8 mL/min. Detection was performed at 245 nm by using PDA detector. The retention times for tenofovir disoproxil fumarate, cobicistat, emtricitabine and elvitegravir were 2.1, 4.3, 5.2 and 8.5 min, respectively. This method was validated for parameters like system suitability, linearity, precision, specificity, accuracy and robustness. This method was successfully applied for the estimation of tenofovir disoproxil fumarate, cobicistat, emtricitabine and elvitegravir in bulk drug and tablet dosage form.

Keywords

Tenofovir disoproxil fumarate Cobicistat Emtricitabine Elvitegravir Gradient elution
Raveendra Babu, V., K. Sharma, P., & Singhvi, I. (2014). A New Gradient Liquid Chromatographic Method for Simultaneous Estimation of Tenofovir Disoproxil Fumarate, Cobicistat, Emtricitabine and Elvitegravir in Bulk Drug and Tablet Dosage Form. Asian Journal of Chemistry, 26(18), 6233–6237. https://doi.org/10.14233/ajchem.2014.17196
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References
  1. S.W. Masho, C.L. Wang and D.E. Nixon, Ther. Clin. Risk Manag., 3, 1097 (2007).
  2. K. Woratanarat, T. Kanjanabuch and C. Suankratay, J. Med. Assoc. Thai., 96, 432 (2013).
  3. Z. Temesgen, Drugs Today, 49, 233 (2013); doi:10.1358/dot.2013.49.4.1947288.
  4. P.L. Anderson, J.J. Kiser, E.M. Gardner, J.E. Rower, A. Meditz and R.M. Grant, J. Antimicrob. Chemother., 66, 240 (2011); doi:10.1093/jac/dkq447.
  5. Z. Temesgen, Drugs Today, 48, 765 (2012); doi:10.1358/dot.2012.48.12.1895682.
  6. J.R. Arribas, J. Eron. Curr. Opin. HIV AIDS, 8, 341 (2013).
  7. J.M. Belavic, Nurse Pract., 38, 24 (2013); doi:10.1097/01.NPR.0000425824.44694.e8.
  8. A. Zolopa, P.E. Sax, E. DeJesus, A. Mills, C. Cohen, D. Wohl, J.E. Gallant, H.C. Liu, A. Plummer, K.L. White, A.K. Cheng, M.S. Rhee and J. Szwarcberg, J. Acquir. Immune Defic. Syndr., 63, 96 (2013); doi:10.1097/QAI.0b013e318289545c.
  9. J.L. Olin, L.M. Spooner and O.M. Klibanov, Ann. Pharmacother., 46, 1671 (2012); doi:10.1345/aph.1R468.
  10. A.A. Shirkhedkar, C.H. Bhirud and S. Sanjaja, Pak. J. Pharm. Sci., 22, 27 (2009).
  11. G. Gnanarajan, A.K. Gupta, V.K. Juyal, P. Yadav and P. Kailash, J. Pharm Anal., 1, 351 (2009).
  12. B. Anindita, P. Aurobinda, K.M. Amit, G.S. Dannana, K.M. Swapan and C. Sudam, Int. J. Pharm. Teach., 3, 1878 (2011).
  13. C.M. Bhaskar Reddy and G.V. Subba Reddy., Int. Res. J. Pharm., 3, 268 (2012).
  14. S.P. Sagar, D. Pratik and M.M. Annapurna, Int. J. Pharm. Res. Dev., 1, 1 (2009).
  15. P.B. Kandagal, D.H. Manjunatha, J. Seetharamappa and S.S. Kalanur, Anal. Lett., 41, 561 (2008); doi:10.1080/00032710801910742.
  16. S. Rajesh and G.A. Pooja, Eurasian J. Anal. Chem., 4, 276 (2009).
  17. S. Padigela, V.V.S. Rajendra Prasad, M.B. Raju and N. Suresh Kumar, Am. J. Pharm. Res., 3, 4697 (2013).
  18. P.D. Hamarapurkar and A.N. Parate, J. Chromatogr. Sci., 51, 419 (2013); doi:10.1093/chromsci/bms157.
  19. P. Kumar, S.C. Dwivedi and A. Kushnoor, Res. J. Chem. Sci., 1, 33 (2007).
  20. A.P. Nikalje, A. Maniyar and S. Obaid, Am. J. PharmTech Res., 3, 651 (2013).
  21. R.K. Valluru and N.B. Kilaru, J. Chromatogr. B, 931, 117 (2013); doi:10.1016/j.jchromb.2013.05.008.
  22. N.A. Gomes, V.V. Vaidya, A. Pudage, S.S. Joshi and S.A. Parekh, J. Pharm. Biomed. Anal., 48, 918 (2008); doi:10.1016/j.jpba.2008.07.022.
  23. W. Kromdijk, S.A. Pereira, H. Rosing, J.W. Mulder, J.H. Beijnen and A.D. Huitema, J. Chromatogr. B, 919-920, 43 (2013); doi:10.1016/j.jchromb.2013.01.005.
  24. S.F.L. Janice, C. Alexandra, A.M. Isabelle and F. Nathan, HIV AIDS, 4, 5 (2012).
  25. ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1) Current Step 4 Version, November (2005).
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