Copyright (c) 2014 AJC
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Phytochemical Screening of Plants Used in Folkloric Medicine: Effect of Extraction Method and Solvent
Corresponding Author(s) : Mazhar Abbas
Asian Journal of Chemistry,
Vol. 26 No. 18 (2014): Vol 26 Issue 18
Abstract
The medicinal plants, Ricinus communis, Croton tiglium and Datura innoxia were screened for phytochemical constituents. The extracting solvents and extraction methods such as shaking, sonication and soxhlet were compared to evaluate their efficiency. The whole plants and plant parts (leaves, stem, root, seed and flower) were extracted with methanol and further fractioned in different solvents. The phytochemical and proximate composition were determined through standard methods. Phytochemical analysis revealed that alkaloids, tannins, saponins, steroids, phlobatannins, flavonoids, terpenoids and C. glycosides were present in the extracts. The quantitative analysis of alkaloids, flavonoids and saponins revealed a considerable variation among plant and parts as well. Furthermore, it was found that extraction method and extracting solvent also affected the phytochemical contents. The moisture, carbohydrate, ash, fat, protein contents and energy value also found to be vary among plants. Results revealed that the plant under investigation have a considerable high amounts of phyto-constituents and a potential sources of new antimicrobial, antioxidant and cytotoxic compounds.
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M. Gulfraz, S. Mehmood, A. Ahmad, N. Fatima, Z. Praveen and E.M. Williamson, Phytother. Res., 22, 1208 (2008); doi:10.1002/ptr.2438.
S.B. Elhardallo, Res. J. Med. Plants, 5, 201 (2011); doi:10.3923/rjmp.2011.201.229.
J. Asghari, B. Ondruschka and M. Mazaheritehrani, J. Med. Plants Res., 5, 495 (2011).
N.K. Kaushik, A. Bagavan, A. Rahuman, D. Mohanakrishnan, C. Kamaraj, G. Elango, A. Zahir and D. Sahal, Exp. Parasitol., 134, 26 (2013); doi:10.1016/j.exppara.2013.01.021.
Z.K. Shinwari, J. Med. Plants Res., 4, 161 (2010).
M. Ibrar, F. Hussain and A. Sultan, Pak. J. Bot., 39, 329 (2007).
P. Tiwari, B. Kumar, M. Kaur, G. Kaur and H. Kaur, Int. Pharma. Sci., 1, 98 (2011).
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E. Menghani, K. Bhatnagar, P. Saraswat and M. Soni, Int. J. Pharm. Res. Develop., 4, 113 (2012).
C.S. Misra, K. Pratyush, M.S.L. Dev, J. James, A.K.T. Veettil and V. Thankamani, Int. J. Resour. Phytochem. Pharmacol., 1, 77 (2011).
S.K. Biswas, A. Chowdhury, J. Das, U.K. Karmakar and M.C. Shill, Int. J. Pharm. Sci. Res., 2, 2605 (2011).
G. Uddin, A. Rauf, T. Rehman and M. Qaisar, Middle-East J. Sci. Res., 7, 707 (2011).
N. Savithramma, M.L. Rao and D. Suhrulatha, Middle-East J. Sci. Res., 8, 579 (2011).
R. Sutharsingh, S. Kavimani, B. Jayakar, M. Uvarani and A. Thangathirupathi, Int. J. Pharm. Stud. Res., 2, 52 (2011).
A.M. Khan, R.A. Qureshi, F. Ullah, S.A. Gilani, A. Nosheen, S. Sahreen, M.K. Laghari, M.Y. Laghari, S. Rehman, I. Hussain and W. Murad, J. Med. Plants Res., 5, 6017 (2011).
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K. Rizwan, M. Zubair, N. Rasool, M. Riaz, M. Zia-Ul-Haq and V. de Feo, Int. J. Mol. Sci., 13, 6440 (2012); doi:10.3390/ijms13056440.
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S.A. Lohlum, African J. Food Agric. Nutri. Develop., 10, 15 (2010).
O.A. Oseni, C.O. Olarinoye and I.A. Amoo, African J. Food Sci., 5, 40 (2011).
B.P. Gaire, R. Lamichhane, C.B. Sunar, A. Shilpakar, S. Neupane and S. Panta, Pharma. J., 3, 1 (2011).
S. Kiruba, M. Mahesh, Z.M. Paul and S. Jeeva, Asian Pacific J. Trop. Biomed., 1, S129 (2011); doi:10.1016/S2221-1691(11)60139-1.
M. Kumar, M. Chandel, M. Kumar and S. Kaur, Phytopharmacol., 1, 177 (2011).
S. Kirmizigul and H. Anil, Turk J. Chem., 26, 947 (2002).