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Pharmacokinetics of Colon-Targeted Pellets of Pulsatilla chinensis (Bunge) Regel Saponins-Hydroxypropyl-b-cyclodextrin Inclusion in Rats
Corresponding Author(s) : Zhenhua Chen
Asian Journal of Chemistry,
Vol. 26 No. 17 (2014): Vol 26 Issue 17
Abstract
The colon-targeted pellets of Pulsatilla chinensis saponins-hydroxypropyl-b-cyclodextrin inclusion was investigated for the pharmacokinetics study in rats. A rapid and sensitive assay was developed and validated using liquid chromatography-mass spectrometry (LC-MS) for Pulsatilla saponin D in rat plasma. Pulsatilla saponin D was extracted with acetonitrile from rat plasma samples and the supernatants was dried in a gentle stream of nitrogen and redissolved with methanol. It was separated using HPLC with a reversed phase column and analyzed by selected ion monitoring (SIM) at m/z of 935.4 and 969.4 for Ginsenoside Re as an internal standard. The Pulsatilla saponin D concentration was measured in plasma samples up to 24 h following oral administration of Pulsatilla chinensis saponins and the colon-targeted pellets of Pulsatilla chinensis saponins-hydroxypropyl-b-cyclodextrin inclusion both at a dose of 200 mg kg-1. The findings indicate that it can decrease the absorption rate and maximum concentration, at the same time improve the bioavailability by preparing colon-targeted pellets of hydroxypropyl-b-cyclodextrin inclusion complex.
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References
Z. Wang, N. Zhang and Y. Sun, Drug Eval. Res., 36, 359 (2013).
J. Hu, J. Practical Trad. Chinese Internal Med., 22, 29 (2008).
W.-C. Ye, N.-N. Ji, S.-X. Zhao, J.-H. Liu, T. Ye, M.A. McKervey and P. Stevenson, Phytochemistry, 42, 799 (1996); doi:10.1016/0031-9422(96)00043-X.
W. Zhang, S. Han and H. Yang, Med. J. West China, 17, 99 (2005).
Y. Zhuo, Pharmacol. Clinics Chinese Mater. Med., 6, 44 (1990).
D.R. Friend, Adv. Drug Deliv., 7, 149 (1991); doi:10.1016/0169-409X(91)90051-D.
Z. Chen and Y. Guan, Chinese J. Exper. Trad. Med. Formulae, 18, 28 (2012).
Z. Chen, Y. Guan and W. Zhu, China J. Chinese Mater. Med., 38, 4292 (2013).