Microbial Community Analysis in Two-Phase Anaerobic Reactor by PCR-Temperature Gradient Gel Electrophoresis

Junhua Fang; Chu Zhang; Maoshen Zhang; Pengcheng Cai

doi:10.14233/ajchem.2014.18160

Issue

Vol. 26 No. 17 (2014): Vol 26 Issue 17

Issue Published : August 27, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Microbial Community Analysis in Two-Phase Anaerobic Reactor by PCR-Temperature Gradient Gel Electrophoresis

https://doi.org/10.14233/ajchem.2014.18160

Junhua Fang

Key Lab of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, P.R. China

Chu Zhang

Key Lab of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, P.R. China

Maoshen Zhang

Xiamen Municipal Engineering Design Institute Co., Ltd. Chongqing Branch, Chongqing 401121, P.R. China

Pengcheng Cai

Chongqing Architectural Design Institute. Municipal Branch, Chongqing 401121, P.R. China

Corresponding Author(s) : Junhua Fang

fangjunhua1126@126.com

Asian Journal of Chemistry, Vol. 26 No. 17 (2014): Vol 26 Issue 17
Article Published : August 12, 2014

Abstract

The seed sludge and the anaerobic sludge for a two-phase anaerobic process that is used for the treatment of N-phenylglycinonitrile wastewater were analyzed by PCR-TGGE technology. Furthermore, the structural differences of the seed sludge and the anaerobic sludge were investigated and compared. Moreover, the Shannon diversity index, an important parameter for studying microbial community diversity, was calculated for each sample. The results showed that the sludge’s microbial community structure was significantly different before and after domestication. The microbial species and structure in the different stages of a two-phase anaerobic reactor were also obviously different. The acidogenic sludge’s diversity index was the highest. Finally, the sludge diversity index of the secondary methanogenic reactor was higher than that of the primary methanogenic reactor and its degradation efficiency of pollutants was also higher.

Keywords

PCR-TGGE Two-phase anaerobic Sludge Microbial community

Fang, J., Zhang, C., Zhang, M., & Cai, P. (2014). Microbial Community Analysis in Two-Phase Anaerobic Reactor by PCR-Temperature Gradient Gel Electrophoresis. Asian Journal of Chemistry, 26(17), 5583–5587. https://doi.org/10.14233/ajchem.2014.18160

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References

J. Chen, S.C. Ma and H.M. Mao, China Anim. Husb. Veterin. Med., 33, 47 (2006).
X.Y. Peng, Y.C. Di, C.X. Jia, B. Mei, L. Wang, J.H. Hong and X.F. Li, Acta Sci. Circumstant., 32, 960 (2012).
K. Calderón, B. Rodelas, N. Cabirol, J. González-López and A. Noyola, Bioresour. Technol., 102, 4618 (2011); doi:10.1016/j.biortech.2011.01.007.
J.Y. Ye, W. Ding and Q. He, Architec. Environ. Eng., 33, 147 (2011).
S.F. Liu, J.J. Fu, L.Y.Li, Foreign Medical Sci., 25,74 (2002).
M.S. Zhang, Study on N-Phenylglycinonitrile Wastewater Treated by Air Stripping-MAP-Two Phase Anaerobic Digestion Process, Chongqing University, p. 12 (2010).
S.G. Shin, G. Han, J. Lim, C. Lee and S. Hwang, Water Res., 44, 4838 (2010); doi:10.1016/j.watres.2010.07.019.
S. Horisawa, Y. Sakuma, Y. Nakamura and S. Doi, Bioresour. Technol., 99, 3084 (2008); doi:10.1016/j.biortech.2007.05.054.
M. Uchida, H. Hatayoshi, A. Syuku-nobe, T. Shimoyama, T. Nakayama, A. Okuwaki, T. Nishino and H. Hemmi, J. Hazard. Mater., 164, 1503 (2009); doi:10.1016/j.jhazmat.2008.09.074.
M. Martins, E.S. Santos, M.L. Faleiro, S. Chaves, R. Tenreiro, R.J. Barros, A. Barreiros and M.C. Costa, Int. Biodeterior. Biodegrad., 65, 972 (2011); doi:10.1016/j.ibiod.2011.07.006.
M. Martins, M.L. Faleiro, S. Chaves, R. Tenreiro, E. Santos and M.C. Costa, J. Hazard. Mater., 176, 1065 (2010); doi:10.1016/j.jhazmat.2009.11.149.
A.A. Abreu, J.C. Costa, P.E.C. Araya-Kroff, E. Ferreira and M. Alves, Water Res., 41, 1473 (2007); doi:10.1016/j.watres.2007.01.017.
C. Malin and P. Illmer, Microbiol. Res., 163, 503 (2008); doi:10.1016/j.micres.2007.07.004.
G.W. Cox and Y.X. Jiang, General Ecology Laboratory Manual, Science Press, pp. 120-124 (1979) (Translation).
R.P. Smyth, T.E. Schlub, A. Grimm, V. Venturi, A. Chopra, S. Mallal, M.P. Davenport and J. Mak, Gene, 469, 45 (2010); doi:10.1016/j.gene.2010.08.009.
C.A. Eichner, R.W. Erb, K.N. Timmis and I. Wagner-Döbler, Appl. Environ. Microbiol., 65, 102 (1999).

References

J. Chen, S.C. Ma and H.M. Mao, China Anim. Husb. Veterin. Med., 33, 47 (2006).

X.Y. Peng, Y.C. Di, C.X. Jia, B. Mei, L. Wang, J.H. Hong and X.F. Li, Acta Sci. Circumstant., 32, 960 (2012).

K. Calderón, B. Rodelas, N. Cabirol, J. González-López and A. Noyola, Bioresour. Technol., 102, 4618 (2011); doi:10.1016/j.biortech.2011.01.007.

J.Y. Ye, W. Ding and Q. He, Architec. Environ. Eng., 33, 147 (2011).

S.F. Liu, J.J. Fu, L.Y.Li, Foreign Medical Sci., 25,74 (2002).

M.S. Zhang, Study on N-Phenylglycinonitrile Wastewater Treated by Air Stripping-MAP-Two Phase Anaerobic Digestion Process, Chongqing University, p. 12 (2010).

S.G. Shin, G. Han, J. Lim, C. Lee and S. Hwang, Water Res., 44, 4838 (2010); doi:10.1016/j.watres.2010.07.019.

S. Horisawa, Y. Sakuma, Y. Nakamura and S. Doi, Bioresour. Technol., 99, 3084 (2008); doi:10.1016/j.biortech.2007.05.054.

M. Uchida, H. Hatayoshi, A. Syuku-nobe, T. Shimoyama, T. Nakayama, A. Okuwaki, T. Nishino and H. Hemmi, J. Hazard. Mater., 164, 1503 (2009); doi:10.1016/j.jhazmat.2008.09.074.

M. Martins, E.S. Santos, M.L. Faleiro, S. Chaves, R. Tenreiro, R.J. Barros, A. Barreiros and M.C. Costa, Int. Biodeterior. Biodegrad., 65, 972 (2011); doi:10.1016/j.ibiod.2011.07.006.

M. Martins, M.L. Faleiro, S. Chaves, R. Tenreiro, E. Santos and M.C. Costa, J. Hazard. Mater., 176, 1065 (2010); doi:10.1016/j.jhazmat.2009.11.149.

A.A. Abreu, J.C. Costa, P.E.C. Araya-Kroff, E. Ferreira and M. Alves, Water Res., 41, 1473 (2007); doi:10.1016/j.watres.2007.01.017.

C. Malin and P. Illmer, Microbiol. Res., 163, 503 (2008); doi:10.1016/j.micres.2007.07.004.

G.W. Cox and Y.X. Jiang, General Ecology Laboratory Manual, Science Press, pp. 120-124 (1979) (Translation).

R.P. Smyth, T.E. Schlub, A. Grimm, V. Venturi, A. Chopra, S. Mallal, M.P. Davenport and J. Mak, Gene, 469, 45 (2010); doi:10.1016/j.gene.2010.08.009.

C.A. Eichner, R.W. Erb, K.N. Timmis and I. Wagner-Döbler, Appl. Environ. Microbiol., 65, 102 (1999).

Issue

Vol. 26 No. 17 (2014): Vol 26 Issue 17

Microbial Community Analysis in Two-Phase Anaerobic Reactor by PCR-Temperature Gradient Gel Electrophoresis

Corresponding Author(s) : Junhua Fang

Abstract

Keywords

Full Article

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