Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals
Corresponding Author(s) : Hayrunnisa Nadaroglu
hnisa25@yahoo.com
Asian Journal of Chemistry,
Vol. 21 No. 7 (2009): Vol 21 Issue 7
Abstract
A peroxidase (POD)-containing fraction was purified from Prangos ferulacea (apiaceae) by (NH4)2SO4 precipitation and anion exchange chromatography. The purification grade obtained was 126.36 yielding 41.8 % of the enzyme activity. The molecular weight was estimated to be 40 kDa by gel filtration. It has an optimum pH of 5 and exhibited high pH and thermal stabilities. Km for guaiacol and ABST were 1.347 and 1 mM, respectively. The optimal temperature was 30 ºC. The I50 values for KSCN and NaN3 were 0.750 mM, 1.321 mM in Prangos ferulacea (Apiaceae) CA, respectively.
Keywords
Enzyme purification
Characterization
Peroxidase
Prangos ferulacea (Apiaceae)
Nadaroglu, H. (2010). Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals. Asian Journal of Chemistry, 21(7), 5768–5776. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/18406
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