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Vol. 30 No. 9 (2018): Vol 30 Issue 9

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Isoflavonoids and Phytoestrogens from Pueraria candollei var. mirifica Related with Appropriate Ratios of Ethanol Extraction

https://doi.org/10.14233/ajchem.2018.21440%20
N. Peerakam
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20130, Thailand
P. Sirisa-Ard
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
N. Quoc Huy
Hanoi University of Pharmacy, Hanoi City, Vietnam
T. Van On
Hanoi University of Pharmacy, Hanoi City, Vietnam
P. Tuan Long
Ministry of Science and Technology (MOST), Hanoi, Vietnam
A. Intharuksa
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand

Corresponding Author(s) : P. Sirisa-Ard

pmpti008@gmail; nichakanp3@gmail.com

Asian Journal of Chemistry, Vol. 30 No. 9 (2018): Vol 30 Issue 9

Abstract

In the folk wisdom of Thailand, chemical compounds from Pueraria candollei var. mirifica play an important role similar to hormones for post-menopausal women. This study aims to determine the appropriate ratio of ethanol to be used for isoflavonoids and phytoestrogens extraction from Pueraria candollei var. mirifica, subsequently, the development as an obstetric cream. Pueraria candollei var. mirifica collected from Thailand and Vietnam were extracted with ethanol (50, 75 and 95 % v/v). The 95 % of ethanolic extract (S095) contained higher quantities of isoflavonoids and phytoestrogens than other ethanolic extracts. The amount of individual isoflavonoids (mg/g extract) were puerarin (5.012), daidzin (2.278), daidzein (1.886), genistin (0.620) and genistein (0.437) which were analyzed by HPLC. The phytoestrogens (mg/g extract) tested by indirect competitive enzyme-linked immunosorbent assay were miroestrol (1.581) and deoxymiroestrol (0.397). In conclusion, 95 % ethanol revealed the highest potential extraction of isoflavonoids and phytoestrogens for use as active ingredients in the development of obstetric creams for post-menopausal women.

Keywords

Pueraria mirifica Isoflavonoids Puerarin Miroestrol Deoxymiroestrol
Peerakam, N., Sirisa-Ard, P., Huy, N. Q., On, T. V., Long, P. T., & Intharuksa, A. (2018). Isoflavonoids and Phytoestrogens from Pueraria candollei var. mirifica Related with Appropriate Ratios of Ethanol Extraction. Asian Journal of Chemistry, 30(9), 2086–2090. https://doi.org/10.14233/ajchem.2018.21440
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