Purification and Properties of Carbonic Anhydrase from Bone Marrow

Abstract

In this work, the carbonic anhydrase was purified from bovine bone marrow and investigated its kinetic properties. Carbonic anhydrase was purified from bovine bone marrow using affinity chromatography by sepharose 4B-L-tyrosine sulphanilamide. During purification steps, the activity of enzyme was measured using p-nitrophenyl acetate at pH: 7.4. Optimum pH and optimum temperature values for bovine bone marrow carbonic anhydrase were determined and then K_M and V_max values for the same substrate were obtained by means of Linewearver-Burk graphics. The purification degree for bovine bone marrow was calculated. The V_max and K_M values at optimum pH and at 20 ºC for the substrate (p-nitrophenyl acetate) were 120.418 μmol/L min and 2.409 × 10^-3 mM, respectively. The K_i and I₅₀ values for sulfanilamide, KSCN, NaN₃ and acetazolamide were determined in bovine bone marrow carbonic anhydrase.