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Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste
Corresponding Author(s) : Stalis Norma Ethica
Asian Journal of Chemistry,
Vol. 30 No. 9 (2018): Vol 30 Issue 9
Abstract
Hydrolytic bacteria are known for their crucial role in the bioconversion of organic matters mainly present in biomedical waste, making them advantageous in bioremediation. Analysis of abundance and colony diversity of culturable bacteria producing hydrolytic enzymes isolated from liquid biomedical waste of two different classes of hospitals in Semarang city, Central Java was carried out. Bacterial cultivation on nutrient agar slants resulted in total 26 viable colonies from inlet samples of Rumah Sakit Umum Roemani Muhammadiyah (R1) and Rumah Sakit Umum Daerah KRMT Wongsonegoro (R2) hospitals. Both hospitals represented two different classes of hospitals in Semarang (C and B, where B class is higher grade). Screening for production of four main hydrolytic enzymes (protease, lipase, amylase and cellulose) on all cultivable colonies obtained in this study. The results showed that all of them were capable of producing more than one type of hydrolytic enzymes. The composition was as follows: Protease producers (26 colonies), amylase producers (14 colonies), cellulose producers (13 colonies) and lipase producers (10 colonies).This study demonstrated that primary inlet of hospitals is a rich source of hydrolytic bacteria, where extracellular protease producers were found to be the main population of this group. In Semarang case, the inlet of lower class of hospital appeared to be more potential source of these hydrolytic bacteria than that of higher class one.
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References
L. Sefouhi, M. Kalla, L. Bahmed and L. Aouragh, Int. J. Environ. Sci. Dev., 4, 442 (2013); https://doi.org/10.7763/IJESD.2013.V4.390.
S.N. Ethica and A. Sabdono, Bio-Remediation Potential of Hydrolytic Bacteria Isolated from Hospital Liquid Biomedical Waste in Central Java, Proceedings of The 3rd World Congress on New Technologies (NewTech’17), Roma, Italy, June 6-8 (2017).
D.R. Sharma, B. Pradhan and S.K. Mishra, Kathmandu Univ. Med. J., 8, 40 (2010).
R.N. Osagie, A.A. Eyaufe and F. Ireye, Int. J. Public Health Sci., 5, 51 (2016).
L. Singhal, A.K. Tuli and V. Gautam, Indian J. Med. Microbiol., 35, 194 (2017).
F. Barancheshme and M. Munir, Front. Microbiol., 8, 2603 (2017); https://doi.org/10.3389/fmicb.2017.02603.
J. Quach-Cu, B. Herrera-Lynch, C. Marciniak, S. Adams, A. Simmerman and R.A. Reinke, Water, 10, 37 (2018); https://doi.org/10.3390/w10010037.
C.C. Azubuike, C.B. Chikere and G.C. Okpokwasili, World J. Microbiol. Biotechnol., 32, 180 (2016); https://doi.org/10.1007/s11274-016-2137-x.
M. Ferrer, O. Golyshina, A. Beloqui and P.N. Golyshin, Curr. Opin. Microbiol., 10, 207 (2007); https://doi.org/10.1016/j.mib.2007.05.004.
S.N. Ethica and T.J. Raharjo, Ph.D. Thesis, Detection of Genes Involved in Glycerol Metabolism of Alcaligenes sp. JG3, Universitas Gadjah Mada, Indonesia (2014).
S.N. Ethica, R. Saptaningtyas, S.I. Muchlissin and A. Sabdono, Health Technol., 1-16 (2018); https://doi.org/10.1007/s12553-018-0232-8.
World Health Organization, Report on Health-Care Waste Management (HCWM) Status in Countries of the South-East Asia Region (2017).
S.N. Ethica, O. Oedjijono, E. Semiarti, J. Widada and T.J. Raharjo, BIOTROPIA-The Southeast Asian J. Trop. Biol., 25, 1 (2018).
O. Pérez-Borla, L.A. Davidovich and S.I. Roura, LWT-Food Chem. Technol., 43, 298 (2010); https://doi.org/10.1016/j.lwt.2009.07.006.
C.A. Mazzucotelli, A.G. Ponce, C.E. Kotlar and M.D. Moreira, Food Sci. Technol., 33, 295 (2013); https://doi.org/10.1590/S0101-20612013005000038.
H. Ariffin, N. Abdullah, M.S.U. Kalsom, Y. Shirai and M.A. Hassan, Int. J. Eng. Technol., 3, 47 (2006); https://doi.org/10.1263/jbb.106.231.
L. Ye and T. Zhang, Appl. Microbiol. Biotechnol., 97, 2681 (2013); https://doi.org/10.1007/s00253-012-4082-4.
V. Chitnis, S. Chitnis, K. Vaidya, S. Ravikant, S. Patil and D.S. Chitnis, Water Res., 38, 441 (2004); https://doi.org/10.1016/j.watres.2003.09.038.
S.N. Ethica, E. Semiarti, J. Widada, O. Oedjijono and T. Joko Raharjo, J. Food Saf., 37, e12345 (2017); https://doi.org/10.1111/jfs.12345.
N. Sonune and A. Garode, Curr. World Environ., 10, 619 (2015); https://doi.org/10.12944/CWE.10.2.27.