Spectrophotometric Determination of Oxidative Coupling Reactions of Nebivolol and Trandolapril

Abstract

Simple and sensitive spectrophotometric methods (M₁-M₃) by the application of oxidative coupling reactions for the assay of nebivolol and trandolapril have been described. Methods M₁, M₂ and M₃ involve the oxidative coupling reactions of nebivolol with (2,3- dimethoxystricychridin- 10-one) (Brucine) (Bru) (Method M₁, λ_max 520 nm) or 3-methyl- 2-benzothiozolinone hydrazone (MBTH) (Method M₂, λ_max 620 nm) in the presence of periodate. Method M₃ is based on the oxidative coupling of trandolapril with Brucine (Method M₃, λ_max 520 nm) in the presence of periodate. Regression analysis of Beer's law plot showed good correlation in the concentration range of 6-36, 2-12, 6-36 μg/mL for methods M₁, M₂ and M₃, respectively. The molar absorptivities lie within the range of 0.0728 × 10⁵–0.2118 × 10⁵ 1 mol^-1 cm^-1 and reproducible with an accuracy of ± 1 %.